Automation solutions can significantly improve sample processing efficiency and can be of particular help in core facility settings. Centralized core facilities are being established world-wide aimed at strengthening institutions' clinical and research enterprises and at addressing the need to process large volumes of samples on expensive cutting-edge technologies in a limited time. High-throughput qPCR profiling is a service offered by most genomics facilities. Several platforms have been developed to process large numbers of samples in a short time, including the Fluidigm Biomark HD, which has also been proved useful to increase the SARS-CoV-2 testing capacities. Several automation systems are currently available to miniaturize volumes and improve bioanalytical workflows, including the SPT Labtech Mosquito HV system. Here we have applied the Mosquito HV platform for the automation of the sample preparation of the Fluidigm gene expression workflow. We have successfully automated the pre-amplification and exonuclease cleanup steps with the aim of reducing manual error and sample processing time. We show consistency in the expression of reference genes when assessing pooled RNA control samples for the manual and automated workflows of Fluidigm gene expression profiling.

中文翻译：

---

当 Mosquito HV 叮咬 Biomark HD：高通量 qPCR 的自动化工作流程。

自动化解决方案可以显着提高样品处理效率，并且在核心设施设置中特别有帮助。全球范围内正在建立集中式核心设施，旨在加强机构的临床和研究企业，并解决在有限时间内使用昂贵的尖端技术处理大量样本的需求。高通量 qPCR 分析是大多数基因组学设施提供的服务。已经开发了几个平台来在短时间内处理大量样本，包括 Fluidigm Biomark HD，它也被证明对提高 SARS-CoV-2 检测能力很有用。目前有几种自动化系统可用于缩小体积并改善生物分析工作流程，包括 SPT Labtech Mosquito HV 系统。在这里，我们将 Mosquito HV 平台应用于 Fluidigm 基因表达工作流程的样品制备自动化。我们已经成功地实现了预扩增和核酸外切酶净化步骤的自动化，旨在减少人工错误和样品处理时间。在为 Fluidigm 基因表达谱的手动和自动工作流程评估合并的 RNA 对照样本时，我们展示了参考基因表达的一致性。