In this study, we used the modified CRISPR/Cas9 system to produce targeted point mutations in cauliflower. Acetolactate synthase (ALS) and Centromere-specific histone H3 variant (CENH3) genes were selected as the base-editing targets and hypocotyls of cauliflower were used as explants. For ALS gene, a C-to-T conversion in the Pro182 codon (CCT) can alter the encoded amino acid, likely resulting in herbicide resistance, and a C-to-T mutation in the Leu133 codon (CTT) in the CENH3 gene may produce a haploid inducer. Results indicated that the transformation efficiency was 1.8%–4.5% and the mutation efficiencies for the ALS and CENH3 genes were approximately 22% and 87%, respectively. The ALS mutant cauliflower showed strong herbicide resistance, with possible immediate implications for broadleaf weed control in cauliflower fields.

在这项研究中，我们使用改良的 CRISPR/Cas9 系统在花椰菜中产生靶向点突变。选择乙酰乳酸合酶（ALS）和着丝粒特异性组蛋白H3变异体（CENH3）基因作为碱基编辑靶点，并以花椰菜的下胚轴作为外植体。对于ALS基因，Pro182 密码子 (CCT) 中的 C 到 T 转换可以改变编码的氨基酸，可能导致除草剂抗性，以及CENH3基因中 Leu133 密码子 (CTT) 中的 C 到 T 突变可能产生单倍体诱导物。结果表明转化效率为 1.8%–4.5%，ALS和CENH3的突变效率为基因分别约为 22% 和 87%。ALS 突变花椰菜表现出很强的除草剂抗性，可能对花椰菜田中的阔叶杂草控制产生直接影响。