Six undescribed compounds, including four undescribed ecdysteroids (cyathsterones A-D) and two undescribed phenolic glycosides (cyathglucosides A-B), were isolated from the roots of Cyathula officinalis Kuan. Their structures were based on chemical analyses, NMR spectroscopic evidence, DP4+ calculations, and hydrolysis products. All compounds inhibited NO release in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages in vitro. Among them, cyathsterone A showed the strongest inhibitory effects. Moreover, cyathsterone A has been shown to inhibit the release of the proinflammatory cytokines TNF-α, IL-6, and IL-1β in LPS-induced RAW 264.7 macrophages in vitro. Further studies found that cyathsterone A present concentration-dependent suppression of the protein expression of iNOS and COX-2 in LPS-stimulated RAW 264.7 cells in vitro and exerted anti-inflammatory activity via the NF-κB signalling pathway.

从Cyathula officinalis Kuan的根中分离出六种未描述的化合物，包括四种未描述的蜕皮激素（cyathsterones AD）和两种未描述的酚苷（cyathglucosides AB） 。它们的结构基于化学分析、NMR 光谱证据、DP4+ 计算和水解产物。所有化合物在体外抑制脂多糖 (LPS) 诱导的 RAW 264.7 巨噬细胞中的 NO 释放。其中，cyathsterone A表现出最强的抑制作用。此外，cyathsterone A 已被证明在体外抑制 LPS 诱导的 RAW 264.7 巨噬细胞中促炎细胞因子 TNF-α、IL-6 和 IL-1β 的释放. 进一步的研究发现，cyathsterone A在体外对LPS 刺激的 RAW 264.7 细胞中 iNOS 和 COX-2 的蛋白质表达存在浓度依赖性抑制，并通过NF-κB 信号通路发挥抗炎活性。