The jasmonic acid (JA) signaling pathway plays a vital role in mediating plant resistance to herbivores. Tea plant (Camellia sinensis) is one of the most important woody cash crops in the world. Due to the lack of genetic transformation systems for tea plants, how the JA signaling pathway works in tea plants has not yet been determined. Now, with the development of cross-disciplines, chemical biology provides new means for analysing the JA signaling pathway. In the present study, the small molecule isoquinoline compound ZINC71820901 (lyn3) was obtained from the ZINC molecular library through virtual screening based on the structure of the crystal COI1-JAZ1 co-receptor and was found to act as an inhibitor of the JA signaling pathway both in Arabidopsis and tea plants. Our results revealed that lyn3 repressed tea plant resistance to Ectropis grisescens mainly by decreasing the accumulation of (-)-epicatechin (EC) and (-)-epigallocatechin (EGC) via repression of the JA signaling pathway, which functioned in the different modulation manner to the already known inhibitor SHAM. As a novel inhibitor of JA signaling pathway, lyn3 provides a specific option for further research on the JA pathway.

中文翻译：

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一种新的 JA 信号通路抑制剂可抑制茶树的食草动物抗性。

茉莉酸 (JA) 信号通路在介导植物对食草动物的抗性中起着至关重要的作用。茶树（Camellia sinensis）是世界上最重要的木本经济作物之一。由于缺乏茶树的遗传转化系统，JA信号通路在茶树中的作用机制尚未确定。现在，随着交叉学科的发展，化学生物学为分析JA信号通路提供了新的手段。本研究基于晶体COI1-JAZ1共受体的结构，通过虚拟筛选从ZINC分子库中获得小分子异喹啉化合物ZINC71820901（lyn3），发现其可作为JA信号通路的抑制剂。在拟南芥和茶树中都有。我们的研究结果表明，lyn3 主要通过抑制 JA 信号通路来减少 (-)-表儿茶素 (EC) 和 (-)-表没食子儿茶素 (EGC) 的积累，从而抑制茶树对灰褐色的抗性，这两种信号通路以不同的调节方式起作用到已知的抑制剂 SHAM。作为一种新型的JA信号通路抑制剂，lyn3为进一步研究JA通路提供了一个特定的选择。