Santa Rosa and Frontier are the major Japanese plum (Prunus salicina Lindl.) cultivars grown throughout the world. The present investigation was performed to understand the genetic relatedness among in vitro propagated plum cultivars Santa Rosa and Frontier using PCR based molecular markers. For the study, three arbitrary markers viz. RAPD (Random amplified Polymorphic DNA), ISSR (Inter-Simple Sequence Repeats) and SCoT (Start Codon Targeted) were used. In RAPD analysis, 18 primers out of 28 amplified and generated 33 scorable bands. The allelic variations when analysed, revealed 84% similarity between these two cultivars with highest polymorphic information content of 0.78. Similarly, 15 ISSR primers produced 73 amplicons with an average of 4.86 amplicon per primer and similarity coefficient ranging from 62 to 67%. Seven SCoT primers out of 26 resulted in a total of twenty- six scorable bands with 24 polymorphic bands. Cluster analysis from all the three markers used broadly divided plum cultivars Santa Rosa and Frontier into two major clusters containing in vitro shoots, their progenies and mother trees of respective genotypes. The study concluded that these three marker systems were found to be effective in revealing genetic relationship of these two commercially important plum cultivars.

Santa Rosa 和 Frontier 是主要的日本李子 ( Prunus salicinaLindl.) 栽培于世界各地的品种。本研究旨在使用基于 PCR 的分子标记了解离体繁殖的李子品种 Santa Rosa 和 Frontier 之间的遗传相关性。对于这项研究，三个任意标记即。使用了 RAPD（随机扩增多态性 DNA）、ISSR（简单序列重复序列间）和 SCoT（靶向起始密码子）。在 RAPD 分析中，28 条引物中的 18 条引物扩增并产生了 33 条可评分条带。分析等位基因变异显示这两个品种之间的相似性为 84%，最高多态信息含量为 0.78。同样，15 个 ISSR 引物产生 73 个扩增子，每个引物平均产生 4.86 个扩增子，相似系数在 62% 到 67% 之间。26 个 SCoT 引物中的 7 个引物产生总共 26 个可评分条带，其中 24 个多态性条带。使用所有三个标记的聚类分析将李子栽培品种 Santa Rosa 和 Frontier 大致分为两个主要聚类，包含体外芽、它们的后代和各自基因型的母树。研究得出结论，发现这三个标记系统可有效揭示这两个具有商业重要性的李子品种的遗传关系。