Anisotropic gold nanomaterials are opening new approaches for the study of cancer cells using high-performance diagnostic devices. Aryldiazonium gold salt, AgNO₃, and ascorbic acid were used as shape-directing agent to promote the growth of spikes over the seeds. We synthesized covalently aryl functionalized gold nanostars via diazonium route using three AgNO₃ concentrations of 1 × 10⁻¹, 5 × 10⁻¹, and 1 × 10⁻⁴ M for spikes length optimization. The seed and growth solutions were mixed and left undisturbed for 24 h. Transmission electron microscopy results showed that the spike sharpness and length were decreased with increasing AgNO₃ concentration. The biocompatible AuNS were incubated with human breast cancer cells MDA-MB-231 and the cellular uptake was estimated using confocal microscopy. Different cancer cell concentrations range of 10–500 cells/mL were used for SERS study. Our results highlight the significance of nano spike-morphology in SERS study on cellular components and efficient cellular uptake of AuNS by MDA-MB-231 compared to normal cells, jointly is important for developing nanomedicines for cancer therapy.

各向异性金纳米材料为使用高性能诊断设备研究癌细胞开辟了新途径。芳基重氮金盐、AgNO ₃和抗坏血酸被用作形状导向剂以促进种子上的穗状花序的生长。我们通过重氮途径合成了共价芳基官能化金纳米星，使用三种浓度为 1 × 10 ^-1、5 × 10 ^-1和 1 × 10 ^-4 M 的AgNO _{3用于尖峰长度优化。}混合种子和生长溶液并静置 24 小时。透射电镜结果表明，随着AgNO _{3的增加，尖峰的锐度和长度减小。}专注。将生物相容性 AuNS 与人乳腺癌细胞 MDA-MB-231 一起孵育，并使用共聚焦显微镜估计细胞摄取。SERS研究使用10-500个细胞/mL的不同癌细胞浓度范围。我们的研究结果突出了纳米尖峰形态在 SERS 研究中对细胞成分的重要性以及 MDA-MB-231 与正常细胞相比对 AuNS 的有效细胞摄取，这对于开发用于癌症治疗的纳米药物很重要。