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The plastome of the husk tomato (Physalis philadelphica Lam., Solanaceae): a comparative analysis between wild and cultivated pools
Genetic Resources and Crop Evolution ( IF 2 ) Pub Date : 2022-01-17 , DOI: 10.1007/s10722-021-01334-x
Isaac Sandoval-Padilla 1 , Jessica Pérez-Alquicira 2, 3 , María del Pilar Zamora-Tavares 3 , Aarón Rodríguez 4 , Ofelia Vargas-Ponce 4
Affiliation  

The fruits of P. philadelphica are of economic value in Mexico. The plants grow wild or cultivated in traditional farming systems. They are also produced as monoculture. Between the wild and cultivated pools, morphological and physiological differences exist. Plant breeding and the shift towards monoculture could modify the plastome sequence and influence the expression of certain genes. Our objectives were threefold. First, we obtained the complete sequence of the domesticated pool plastome. Then, we compared this to the plastome from the wild pool. Finally, we identified the changes that could be related to the domestication process. The plastome of the Manzano race was sequenced, characterized and compared with the plastome of the Milpero (wild) race. The plastomes had the typical circular and quadripartite structure but differed by 52 bp (domesticated 156,856 bp, wild 156,804 bp). A total of 115 genes and five pseudogenes were identified. In addition, 29 SNPs were detected in coding regions along with 19 polymorphic microsatellites. The complete sequences were highly conserved with an identity of 99.6%. No major differences were found in the coding regions that could be attributed to selection and the change from wild to monoculture. InDels in non-coding regions produced more variation. The variation observed was similar to that detected in other managed species.



中文翻译:

果壳番茄(酸浆 philadelphica Lam.,茄科)的质体:野生和栽培池之间的比较分析

P. philadelphica的果实在墨西哥具有经济价值。这些植物在传统农业系统中野生或栽培。它们也作为单一栽培生产。野生和养殖池之间存在形态和生理差异。植物育种和向单一栽培的转变可能会改变质体序列并影响某些基因的表达。我们的目标是三重的。首先,我们获得了驯化池质体的完整序列。然后,我们将其与野生池中的塑性体进行了比较。最后,我们确定了可能与驯化过程有关的变化。对Manzano种族的质体进行测序、表征并与Milpero的质体进行比较(野)种族。质体具有典型的圆形和四分体结构,但相差 52 bp(驯化 156,856 bp,野生 156,804 bp)。共鉴定出115个基因和5个假基因。此外,在编码区检测到 29 个 SNP 以及 19 个多态性微卫星。完整序列高度保守,同一性为99.6%。在编码区域中没有发现可归因于选择和从野生到单一栽培的变化的重大差异。非编码区的 InDels 产生了更多的变异。观察到的变化与在其他管理物种中发现的相似。

更新日期:2022-01-17
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