Root-knot nematodes are among the most dangerous plant pathogens. Biological control is a safer and effective way to manage such pests. In this context, bacterial strain KMS-6 was isolated from nematode-affected soil of the research field, CCS HAU, Hisar. It was subjected to morphological and biochemical characterization. The bacterium produced 14.02 ± 0.03 µg/ml of IAA and showed a cyanogenic activity. KMS-6 was identified based on 16S rRNA gene sequence analysis as Bacillus altitudinis (accession no. MT626660). In vitro experiments with fermentation supernatants of KMS-6 resulted in potential hatching inhibition and up to 86% J2s mortality of M. javanica. The bacterium significantly suppressed root-knot nematode infestations in greenhouse and field experiments. During the greenhouse experiment on eggplant, KMS-6 inoculation resulted in a 76% reduction in eggs, 80% reduction in galls, reducing the final nematode population up to 92% compared to control. All trials were significantly more effective than the chemical treatment as well. Also, an enhancement in plant growth was observed in KMS-6 inoculated plants with the highest plant height, maximum fresh and dry weight of root and shoot. Similar results were seen in trials conducted on eggplant (2018) and cucumber (2019) crops in nematode-infested fields. KMS-6 inoculation reduced nematode infestation more effectively than control and carbofuran treatments. The yield was significantly improved in the KMS-6 treatment. Data suggest that B. altitudinis KMS-6 could be an effective biological control agent with plant-growth enhancing properties against root-knot nematodes and a potent alternative to chemical nematicides.

根结线虫是最危险的植物病原体之一。生物防治是管理此类害虫的一种更安全、更有效的方法。在此背景下，从研究领域 CCS HAU、Hisar 的受线虫影响的土壤中分离出菌株 KMS-6。对其进行形态学和生化表征。该细菌产生 14.02 ± 0.03 µg/ml IAA 并显示出氰活性。基于 16S rRNA 基因序列分析，KMS-6 被鉴定为Bacillus altitudinis（登录号 MT626660）。KMS-6 发酵上清液的体外实验导致爪哇爪哇潜在的孵化抑制和高达 86% 的 J2s 死亡率. 该细菌在温室和田间试验中显着抑制了根结线虫的侵扰。在茄子温室实验期间，与对照相比，接种 KMS-6 导致卵减少 76%，虫瘿减少 80%，最终线虫种群减少高达 92%。所有试验也明显比化学治疗更有效。此外，在具有最高株高、最大鲜重和干重的根和枝条的 KMS-6 接种植物中观察到植物生长的增强。在受线虫感染的田地中对茄子（2018 年）和黄瓜（2019 年）作物进行的试验中也看到了类似的结果。KMS-6 接种比对照和克百威处理更有效地减少了线虫侵染。KMS-6处理的收率显着提高。数据表明B. altitudinis KMS-6 可能是一种有效的生物控制剂，具有促进植物生长的特性，可对抗根结线虫，是化学杀线虫剂的有效替代品。