The effect of functionalization of anodized zirconium (Zr) with RGD peptides on cell behavior as a feasible approach to stimulate early bone integration is presented. The surface modifications were evaluated by Raman spectroscopy and X-ray photoelectron spectroscopy (XPS). Electrochemical tests were performed to determine the effect of functionalization on the stability of the anodized zirconium oxide film. Finally, the biocompatibility of the material was evaluated in an in vitro cell model. Surface analysis showed the presence of peptides on the surface after functionalization, and that the crystal structure of the oxide formed by anodization is predominantly monoclinic. The immobilization of RGD peptides on anodized Zr does not alter the corrosion protection provided by the anodic oxide and significantly promote cell adhesion and spreading in vitro. Thus, the combination of RGD peptides with anodic oxide on Zr surface would represent a useful strategy to increases the biocompatibility of the material.

提出了用 RGD肽对阳极氧化锆 (Zr) 进行功能化对细胞行为的影响，作为刺激早期骨整合的可行方法。通过拉曼光谱和 X 射线光电子能谱 (XPS) 评估表面改性。进行电化学测试以确定功能化对阳极氧化锆膜稳定性的影响。最后，在体外评估材料的生物相容性细胞模型。表面分析表明，功能化后表面存在肽，阳极氧化形成的氧化物的晶体结构主要为单斜晶系。将 RGD 肽固定在阳极氧化的 Zr 上不会改变阳极氧化物提供的腐蚀保护，并显着促进细胞在体外的粘附和扩散。因此，RGD 肽与 Zr 表面上的阳极氧化物的组合将代表一种提高材料生物相容性的有用策略。