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Regeneration of Plants from Alginate-encapsulated Shoot Tips of Myrtle (Myrtus communis L.)
Erwerbs-Obstbau ( IF 1.3 ) Pub Date : 2022-01-10 , DOI: 10.1007/s10341-021-00632-z
Dicle Dönmez 1
Affiliation  

Myrtle growing naturally in the Mediterranean Region in Turkey, is among the economically important plant species. The present study emphasized on in vitro conservation of M. communis by encapsulating regenerated shoot tips. In this research we reported synthetic seed production and subsequent conversion of encapsulated shoot tips into plantlets comparing with nonencapsulated shoot tips for myrtle. Two different myrtle genotypes were used for synthetic seed production. Sodium alginate solution at the rate of 3.0% and 100 mM calcium chloride solution were prepared for encapsulation. Encapsulation was accomplished by mixing shoot tips into sodium alginate solution and dropping these in calcium chloride solution for 25–30 min. Encapsulated and nonencapsulated shoot tips were cultured in MS (Murashige and Skoog) media supplemented with different BAP (6-Benzylaminopurine) concentrations (0, 0.5, 1, 2 mg L−1). After six weeks, all shoots were transferred to MS media containing 1 mg L−1 IBA for in vitro rooting. As a result, the highest germination rate was obtained on the BAP-free MS media. The best BAP concentrations were detected as 0.5 and 1 mg L−1 for micropropagation. Genetic stability of plants coming from encapsulated and nonencapsulated shoot tips was tested by ISSR markers. Based on the results, there were no genetic differences among the samples.



中文翻译:

用藻酸盐包封的桃金娘芽尖再生植物

在土耳其地中海地区自然生长的桃金娘是经济上重要的植物物种之一。本研究着重于M. Communis 的体外保存通过封装再生的芽尖。在这项研究中,我们报告了合成种子的生产以及随后将包封的茎尖转化为小植物,与桃金娘的非包膜茎尖相比。两种不同的桃金娘基因型用于合成种子生产。制备比例为 3.0% 的海藻酸钠溶液和 100 mM 氯化钙溶液用于封装。通过将芽尖混合到海藻酸钠溶液中并将它们滴入氯化钙溶液中 25-30 分钟来完成封装。在补充有不同 BAP (6-苄氨基嘌呤) 浓度 (0, 0.5, 1, 2 mg L -1 ) 的MS (Murashige 和 Skoog) 培养基中培养封装和未封装的芽尖。六周后,将所有枝条转移到含有 1 mg L-1 IBA 用于体外生根。结果,在不含 BAP 的 MS 培养基上获得了最高的发芽率。最佳 BAP 浓度被检测为 0.5 和 1 mg L -1用于微繁殖。通过 ISSR 标记测试来自包膜和非包膜茎尖的植物的遗传稳定性。根据结果​​,样本之间没有遗传差异。

更新日期:2022-01-11
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