Prunus napaulensis (Ser.) Steud. is one of the important indigenous fruit trees of North-eastern India found in wild under niche habitat, facing immense exploitation due to its medicinally important fruits. Detailed studies on seed storage biology are required for devising appropriate ex situ and in situ conservation strategies of this species. Accordingly, desiccation and freezing tolerance were investigated in seeds and excised embryonic axes (EA) for standardizing long-term conservation through cryopreservation. The large-sized fresh seeds with 46% moisture content (MC) were found highly sensitive to desiccation, with 80% loss in viability when desiccated to 30% MC. In contrast, the freshly isolated embryonic axes (49% MC) showed remarkable tolerance when desiccated to 11% MC and yielded high recovery (81%) after cryopreservation when cultured on Murashige and Skoog medium with 0.1 mgL⁻¹ 6-benzylaminopurine and 0.05 mgL⁻¹ naphthaleneacetic acid. Viability reduced significantly when EA were dehydrated below critical MC (11%) both in desiccated and cryopreserved embryos. Differential response of tissues in the EA was also recorded, with radical exhibiting high degree of desiccation and freezing sensitivity. Excised EA when cultured in vitro formed shoots but failed to develop roots. This study demonstrates that P. napaulensis seeds have recalcitrant storage behaviour and cryoprotection would be required for ex situ germplasm conservation.

核桃（系列）斯图德。是印度东北部重要的本土果树之一，在生态位栖息地的野外发现，由于其重要的药用果实而面临巨大的开发。需要对种子储存生物学进行详细研究，以制定适当的该物种的异地和就地保护策略。因此，研究了种子和切除胚轴 (EA) 的干燥和耐冻性，以通过冷冻保存标准化长期保存。发现含水量 (MC) 为 46% 的大型新鲜种子对干燥高度敏感，当干燥至 30% MC 时，活力损失 80%。相比之下，^-1 6-苄基氨基嘌呤和 0.05 mgL ^-1萘乙酸。当 EA 在干燥和冷冻保存的胚胎中脱水低于临界 MC (11%) 时，活力显着降低。还记录了 EA 中组织的差异反应，自由基表现出高度的干燥和冷冻敏感性。体外培养时切除的 EA 形成枝条，但未能形成根。这项研究表明，P. napaulensis种子具有顽固的储存行为，并且异地种质保存需要冷冻保护。