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Changes in the gut bacterial communities in colon cancer surgery patients: an observational study
Gut Pathogens ( IF 4.2 ) Pub Date : 2022-01-04 , DOI: 10.1186/s13099-021-00477-7
Mohamed Abbas 1 , Nadia Gaïa 2 , Nicolas C Buchs 3 , Vaihere Delaune 3 , Myriam Girard 2 , Diego O Andrey 4 , Jeremy Meyer 3 , Jacques Schrenzel 2, 4, 5 , Frédéric Ris 3 , Stephan Harbarth 1, 4 , Vladimir Lazarevic 2
Affiliation  

Colon surgery has been shown to modulate the intestinal microbiota. Our objective was to characterize these changes using state-of-the-art next generation sequencing techniques. We performed a single-centre prospective observational cohort study to evaluate the changes in the gut microbiota, i.e., taxon distribution, before and after elective oncologic colon surgery in adult patients with different antimicrobial prophylaxis regimens (standard prophylaxis with cefuroxime/metronidazole versus carbapenems for extended-spectrum beta-lactamase-producing Enterobacterales [ESBL-E] carriers). We obtained rectal samples on the day of surgery, intraoperative luminal samples, and rectal or stoma samples 3 days after surgery. We performed metataxonomic analysis based on sequencing of the bacterial 16S rRNA gene marker. Similarities and differences between bacterial communities were assessed using Bray–Curtis similarity, visualised using principal coordinates analysis and statistically tested by PERMANOVA. Comparison of taxa relative abundance was performed using ANCOM. We included 27 patients between March 27, 2019 and September 17, 2019. The median age was 63.6 years (IQR 56.4–76.3) and 44% were females. Most (81%) patients received standard perioperative prophylaxis as they were not ESBL carriers. There was no significant association between ESBL carriage and differences in gut microbiome. We observed large and significant increases in the genus Enterococcus between the preoperative/intraoperative samples and the postoperative sample, mainly driven by Enterococcus faecalis. There were significant differences in the postoperative microbiome between patients who received standard prophylaxis and carbapenems, specifically in the family Erysipelotrichaceae. This hypothesis-generating study showed rapid changes in the rectal microbiota following colon cancer surgery.

中文翻译:

结肠癌手术患者肠道细菌群落的变化:一项观察性研究

结肠手术已被证明可以调节肠道微生物群。我们的目标是使用最先进的下一代测序技术来表征这些变化。我们进行了一项单中心前瞻性观察性队列研究,以评估接受不同抗菌预防方案(头孢呋辛/甲硝唑标准预防与碳青霉烯类延长产β-内酰胺酶的肠杆菌[ESBL-E] 携带者)。我们在手术当天获得直肠样本、术中管腔样本以及手术后 3 天的直肠或造口样本。我们基于细菌 16S rRNA 基因标记的测序进行了元分类学分析。使用 Bray-Curtis 相似性评估细菌群落之间的相似性和差异,使用主坐标分析可视化并通过 PERMANOVA 进行统计测试。使用 ANCOM 进行分类群相对丰度的比较。我们纳入了 2019 年 3 月 27 日至 2019 年 9 月 17 日期间的 27 名患者。中位年龄为 63.6 岁(IQR 56.4-76.3),44% 为女性。大多数(81%)患者接受标准的围手术期预防,因为他们不是 ESBL 携带者。ESBL 携带与肠道微生物组差异之间没有显着关联。我们观察到术前/术中样本和术后样本之间肠球菌属的显着增加,主要由粪肠球菌驱动。接受标准预防和碳青霉烯类药物治疗的患者术后微生物组存在显着差异,特别是在丹毒科。这项产生假设的研究表明,结肠癌手术后直肠微生物群发生了快速变化。
更新日期:2022-01-05
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