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Evaluation of molecular and morphological diversity of caper (Capparis spinosa L.)
Genetic Resources and Crop Evolution ( IF 2 ) Pub Date : 2022-01-04 , DOI: 10.1007/s10722-021-01315-0
Noshin Mahmodi 1 , Gholam-Reza Sharifi-Sirchi 2 , Kianoosh Cheghamirza 3
Affiliation  

The objective of this study was the evaluation of molecular and morphological diversity among 80 caper (Capparis spinosa L.) genotypes from the 12 regions of the central Zagros Mountains located in the west of Iran. The results showed a high level of morphological genetic variation among the caper samples. According to the morphological cluster analysis, 80 genotypes were clustered into five main groups. The 15 factors justified 78.7% of the total variation based on factor analysis. ISSR primers produced a total of 108 polymorphic bands (85.04%) from 127 bands and the PIC for primers ranged from 0.02 to 0.33. SCoT primers produced a total of 165 polymorphic bands (86.48%) from 190 bands and the PIC for primers ranged from 0.16 to 0.30. Ordination and cluster analysis by ISSR markers showed that the genetic relationships among all accessions could be separated into three major groups and by SCoT markers separated into six groups. The results did not show a perfect match between the molecular diversity groupings and geographical regions, because many natural factors and human activities shape the amount and pattern of genetic diversity in a plant population. SCoT and ISSR markers for the assessment of genetic diversity of caper germplasm were almost identical. The combined (ISSR + SCoT) markers haven't shown more information on genetic diversity than a single analysis of ISSR and SCoT. The results indicated the existence of dispersion and different levels of morphological variation and molecular genetic diversity in the genotypes collected from the west of Iran.



中文翻译:

刺山柑(Capparis spinosa L.)的分子和形态多样性评价

本研究的目的是评估 80刺山柑Capparis spinosaL.) 来自伊朗西部扎格罗斯山脉中部 12 个地区的基因型。结果表明,雀跃样品中存在高度的形态遗传变异。根据形态学聚类分析,80个基因型被分为五个主要组。根据因子分析,这 15 个因子证明了总变异的 78.7%。ISSR 引物从 127 个条带中总共产生了 108 个多态性条带(85.04%),引物的 PIC 范围从 0.02 到 0.33。SCoT 引物从 190 个条带中总共产生了 165 个多态性条带(86.48%),引物的 PIC 范围从 0.16 到 0.30。ISSR标记排序和聚类分析表明,所有种质之间的亲缘关系可以分为三大类,SCoT标记可以分为六类。结果没有显示分子多样性分组和地理区域之间的完美匹配,因为许多自然因素和人类活动塑造了植物种群遗传多样性的数量和模式。用于评估刺山柑种质遗传多样性的 SCoT 和 ISSR 标记几乎相同。组合 (ISSR + SCoT) 标记没有显示出比 ISSR 和 SCoT 的单一分析更多的遗传多样性信息。结果表明,从伊朗西部采集的基因型存在分散性和不同程度的形态变异和分子遗传多样性。用于评估刺山柑种质遗传多样性的 SCoT 和 ISSR 标记几乎相同。组合 (ISSR + SCoT) 标记没有显示出比 ISSR 和 SCoT 的单一分析更多的遗传多样性信息。结果表明,从伊朗西部采集的基因型存在分散性和不同程度的形态变异和分子遗传多样性。用于评估刺山柑种质遗传多样性的 SCoT 和 ISSR 标记几乎相同。组合 (ISSR + SCoT) 标记没有显示出比 ISSR 和 SCoT 的单一分析更多的遗传多样性信息。结果表明,从伊朗西部采集的基因型存在分散性和不同程度的形态变异和分子遗传多样性。

更新日期:2022-01-05
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