Liquid chromatography/tandem mass spectrometry is firmly established today as the gold standard technique for analysis of vitamin D, both for vitamin D status assessments as well as for measuring complex and intricate vitamin D metabolic fingerprints. While the actual mass spectrometry technology has seen only incremental performance increases in recent years, there have been major, very impactful changes in the front- and back-end of MS-based vitamin D assays; for example, the extension to new types of biological sample matrices analyzed for an increasing number of different vitamin D metabolites, novel sample preparation techniques, new powerful chemical derivatization reagents, as well the continued integration of high resolution mass spectrometers into clinical laboratories, replacing established triple-quadrupole instruments. At the same time, the sustainability of mass spectrometry operation in the vitamin D field is now firmly established through proven analytical harmonization and standardization programs. The present review summarizes the most important of these recent developments.

中文翻译：

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通过质谱法分析维生素 D 代谢标志物：“金标准”方法及其融入临床实践的最新进展

液相色谱/串联质谱如今已成为维生素 D 分析的黄金标准技术，既可用于维生素 D 状态评估，也可用于测量复杂的维生素 D 代谢指纹。虽然近年来实际的质谱技术性能仅有所提高，但基于 MS 的维生素 D 测定的前端和后端发生了重大且非常有影响力的变化；例如，扩展到新型生物样品基质，分析越来越多的不同维生素 D 代谢物、新颖的样品制备技术、新型强大的化学衍生化试剂，以及将高分辨率质谱仪持续集成到临床实验室中，取代现有的质谱仪。三重四极杆仪器。与此同时，维生素 D 领域质谱操作的可持续性现已通过经过验证的分析协调和标准化计划得到牢固确立。本综述总结了这些最新进展中最重要的部分。