Clinical Oral Implants Research ( IF 4.3 ) Pub Date : 2021-12-28 , DOI: 10.1111/clr.3_13855
Yuki Ichioka1,*; Jan Derks1; Gunnar Dahrén2; Tord Berglundh1; Lena Larsson1
1Periodontology; 2Oral Microbiology and Immunology, University of Gothenburg, Gothenburg, Sweden
Background: Removal of bacterial biofilm and reestablishing biocompatibility of implant surfaces are critical components of peri-implantitis therapy. Optimal mechanical protocols for surface decontamination are yet to be identified.
Aim/Hypothesis: The objective of this in-vitro study was to evaluate surface cleanness and cytocompatibility following mechanical decontamination procedures on biofilm-coated titanium discs.
Material and Methods: Sterile titanium discs with non-modified and modified surfaces were contaminated with Streptococcus gordonii. Decontamination was performed through four different mechanical procedures; i) gauze soaked in sterile saline, ii) ultra-sonic device with a PEEK fiber tip, iii) rotating nickel-titanium brush, or iv) air-polishing device. SEM was used to identify residual deposits (bacteria, foreign material and spot-like deposits) following decontamination using SE2 and backscattered electron detectors. Cytocompatibility of treated titanium discs was assessed for osteoblast-like cells using immunofluorescence and SEM. Cells attached on the treated discs were stained with anti-vinculin monoclonal antibody, rhodamine phalloidin and Hoechst. The mean % area of cells and bacteria was evaluated. Non-contaminated, untreated titanium disks were used as controls.
Results: Mechanical instrumentation of discs with a non-modified surface resulted in an almost complete absence of residual bacteria in all groups. Air polishing was superior to the other treatment groups in removing bacteria from modified surface discs. Cleanness was significantly influenced by implant surface characteristics in all groups with the exception of air polishing. Gauze remnants and plastic deposits were detected on treated titanium discs in the respective groups (cleaning by gauze or PEEK fiber tip). Both the use of a rotating nickel-titanium brush and air polishing was superior to other methods in restoring cytocompatibility although no method of mechanical decontamination resulted in cytocompatibility levels of pristine titanium surfaces.
Conclusion and Clinical implications: Air-polishing was superior to other mechanical methods in terms of cleaning capacity and restoration of cytocompatibility. Based on the present in-vitro findings, the cleaning potential of air-polishing devices in clinical situations should be evaluated in randomized control clinical trials.
Disclosure of Interest: Y. Ichioka: None Declared, J. Derks Conflict with: Dentsply implants, Conflict with: Dentsply implants, Straumann, Conflict with: Dentsply implants, Straumann, G. Dahrén: None Declared, T. Berglundh Conflict with: University grant, Dentsply Implants, Conflict with: Dentsply implants, L. Larsson: None Declared.
Keywords: biocompatibility, implant surface, peri-implantitis
中文翻译:
EAO-172 / OC-BR-004 | 生物膜涂层钛圆盘的仪器
Yuki Ichioka 1,* ; 扬·德克斯1 ; Gunnar Dahrén 2 ; 托德·伯格伦德1 ; 莉娜·拉尔森1
1牙周病学;2瑞典哥德堡哥德堡大学口腔微生物学和免疫学
背景:去除细菌生物膜和重建种植体表面的生物相容性是种植体周围炎治疗的关键组成部分。表面去污的最佳机械协议尚待确定。
目的/假设:这项体外研究的目的是评估生物膜涂层钛圆盘机械去污程序后的表面清洁度和细胞相容性。
材料和方法:具有未改性和改性表面的无菌钛盘被戈登链球菌污染. 去污通过四种不同的机械程序进行;i) 浸泡在无菌盐水中的纱布,ii) 带有 PEEK 纤维尖端的超声波装置,iii) 旋转镍钛刷,或 iv) 空气抛光装置。SEM 用于在使用 SE2 和背散射电子检测器去污后识别残留沉积物(细菌、异物和斑点状沉积物)。使用免疫荧光和扫描电镜评估处理过的钛椎间盘的细胞相容性,以获得成骨细胞样细胞。附着在处理过的椎间盘上的细胞用抗纽蛋白单克隆抗体、罗丹明鬼笔环肽和 Hoechst 染色。评估细胞和细菌的平均百分比面积。未受污染、未经处理的钛盘用作对照。
结果:具有未改性表面的圆盘的机械仪器导致所有组中几乎完全没有残留细菌。空气抛光在去除改性表面盘上的细菌方面优于其他处理组。除空气抛光外,所有组的种植体表面特征均显着影响清洁度。在各组中处理过的钛盘上检测到纱布残留物和塑料沉积物(用纱布或 PEEK 纤维尖端清洁)。使用旋转镍钛刷和空气抛光在恢复细胞相容性方面都优于其他方法,尽管没有任何机械去污方法导致原始钛表面的细胞相容性水平。
结论和临床意义:空气抛光在清洁能力和细胞相容性恢复方面优于其他机械方法。根据目前的体外研究结果,应在随机对照临床试验中评估临床情况下空气抛光设备的清洁潜力。
利益披露:Y. Ichioka:无声明,J. Derks 与:Dentsply 植入物冲突,与:Dentsply 植入物,Straumann 冲突,与:Dentsply 植入物,Straumann,G. Dahrén:未声明,T. Berglundh 冲突:大学授予,登士柏种植体,与:登士柏种植体,L. Larsson 的冲突:无声明。
关键词: 生物相容性, 种植体表面, 种植体周围炎