Cell cycle regulation plays a critical role in plant growth and development. In this study, the role of a tomato cell cycle gene SlCycB1 has been characterized. Expression analysis revealed that SlCycB1 was mostly expressed in stem, root, and leaves, with relative lower expression in flower and fruit. Tomato plants overexpressing SlCycB1 exhibited a reduction in cell number and increased cell size leading to the growth retardation. Furthermore, yeast two-hybrid analysis and bimolecular fluorescence complementation revealed that SlCycB1 interacted with histone H3.2, an essential component of the nucleosome. Histone H3.2 was transcriptionally up-regulated in the SlCycB1 overexpressing tomato lines. Furthermore, the overexpression of histone H3.2 in transgenic plants showed similar phenotypes to SlCycB1 overexpressing lines. Based on these findings, we concluded that SlCycB1 overexpression altered tomato architecture in association with histone H3.2.

细胞周期调控在植物生长发育中起着至关重要的作用。在这项研究中，番茄细胞周期基因SlCycB1的作用已经得到表征。表达分析表明，SlCycB1主要在茎、根和叶中表达，在花和果实中表达相对较低。过表达SlCycB1的番茄植株表现出细胞数量减少和细胞大小增加导致生长迟缓。此外，酵母双杂交分析和双分子荧光互补表明，SlCycB1 与组蛋白 H3.2 相互作用，组蛋白 H3.2 是核小体的重要组成部分。组蛋白 H3.2在SlCycB1中被转录上调过度表达番茄线。此外，组蛋白 H3.2在转基因植物中的过表达显示出与SlCycB1过表达系相似的表型。基于这些发现，我们得出结论，SlCycB1过表达改变了与组蛋白 H3.2相关的番茄结构。