Proteases cut with enviable precision and regulate diverse molecular events in biology. Such qualities drive a seemingly inexhaustible appetite for proteases with new activities and capabilities. Comprising 25% of the total industrial enzyme market, proteases appear in consumer goods, such as detergents, textile processing, and numerous foods; additionally, proteases include 25 US Food and Drug Administration-approved medicines and various research tools. Recent advances in protease engineering strategies address target specificity, catalytic efficiency, and stability. This guide to protease engineering surveys best practices and emerging strategies. We further highlight gaps and flexibilities inherent to each system that suggest opportunities for new technology development along with engineered proteases to solve challenges in proteomics, protein sequencing, and synthetic gene circuits.

蛋白酶以令人羡慕的精度切割并调节生物学中的各种分子事件。这些品质驱使人们对具有新活性和新功能的蛋白酶产生看似无穷无尽的胃口。占整个工业酶市场的 25%，蛋白酶出现在消费品中，例如洗涤剂、纺织品加工和许多食品；此外，蛋白酶包括 25 种美国食品和药物管理局批准的药物和各种研究工具。蛋白酶工程策略的最新进展解决了目标特异性、催化效率和稳定性问题。本指南介绍了蛋白酶工程调查的最佳实践和新兴策略。我们进一步强调了每个系统固有的差距和灵活性，这表明新技术开发的机会以及工程化蛋白酶以解决蛋白质组学中的挑战，