The incidence of cutaneous squamous cell carcinoma (cSCC) has been increasing in recent years. Meanwhile, microRNAs (miRNAs) have been found to play vital roles in various cancers, including cSCC. This study aimed to investigate the expression of microRNA-573 (miR-573) in cSCC, its relationship with long non-coding RNA PICSAR and analyze its biological role. The relationship between PICSAR and miR-573 was confirmed by dual-luciferase reporter assay and Pearson's correlation coefficient analysis. The levels of PICSAR and miR-573 were measured using quantitative Real-Time PCR. Cell Counting Kit-8 assay was used to evaluate the cSCC cell proliferation ability. The migration and invasion abilities of cSCC cells were evaluated by Transwell assay. PICSAR expression was increased and miR-573 was decreased in tumor tissues and cSCC cell lines. PICSAR and miR-573 can bind directly, and miR-573 expression was downregulated by PICSAR in cSCC. Overexpression of miR-573 significantly inhibited the proliferation, migration and invasion abilities of A431 and SCC13 cells. Additionally, miR-573 overexpression reversed the promotion effects of PICSAR overexpression on cSCC cell proliferation, migration and invasion abilities. In conclusion, our findings indicated that miR-573 expression was decreased in tumor tissues and cSCC cells and was downregulated by PICSAR in cSCC. Additionally, miR-573 overexpression inhibited cSCC cell proliferation, migration and invasion, and reversed the promotion effects of PICSAR overexpression on cSCC cell biological functions. Thus, miR-573 might function as a tumor suppressor and might be involved in the regulatory effects of PICSAR on tumorigenesis in cSCC.

中文翻译：

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MicroRNA-573 在皮肤鳞状细胞癌中抑制细胞增殖、迁移和侵袭，并被 PICSAR 下调。

近年来，皮肤鳞状细胞癌（cSCC）的发病率呈上升趋势。同时，已发现 microRNA (miRNA) 在包括 cSCC 在内的各种癌症中发挥重要作用。本研究旨在探讨microRNA-573（miR-573）在cSCC中的表达及其与长链非编码RNA PICSAR的关系，并分析其生物学作用。PICSAR 和 miR-573 之间的关系通过双荧光素酶报告基因分析和 Pearson 相关系数分析得到证实。使用定量实时 PCR 测量 PICSAR 和 miR-573 的水平。Cell Counting Kit-8 测定用于评估 cSCC 细胞增殖能力。通过 Transwell 试验评估 cSCC 细胞的迁移和侵袭能力。在肿瘤组织和 cSCC 细胞系中 PICSAR 表达增加，miR-573 减少。PICSAR 和 miR-573 可以直接结合，并且 miR-573 在 cSCC 中的表达被 PICSAR 下调。miR-573的过表达显着抑制A431和SCC13细胞的增殖、迁移和侵袭能力。此外，miR-573 过表达逆转了 PICSAR 过表达对 cSCC 细胞增殖、迁移和侵袭能力的促进作用。总之，我们的研究结果表明，miR-573 在肿瘤组织和 cSCC 细胞中的表达降低，并且在 cSCC 中被 PICSAR 下调。此外，miR-573过表达抑制cSCC细胞增殖、迁移和侵袭，并逆转PICSAR过表达对cSCC细胞生物学功能的促进作用。因此，miR-573 可能作为肿瘤抑制因子发挥作用，并可能参与 PICSAR 对 cSCC 肿瘤发生的调节作用。