当前位置: X-MOL 学术Plant Biotech. J. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
TaMOR is essential for root initiation and improvement of root system architecture in wheat
Plant Biotechnology Journal ( IF 13.8 ) Pub Date : 2021-12-10 , DOI: 10.1111/pbi.13765
Chaonan Li 1 , Jingyi Wang 1 , Long Li 1 , Jialu Li 1 , Mengjia Zhuang 1 , Bo Li 1 , Qiaoru Li 1 , Junfang Huang 1 , Yan Du 1 , Jinping Wang 1 , Zipei Fan 1 , Xinguo Mao 1 , Ruilian Jing 1
Affiliation  

Optimal root system architecture is beneficial for water-fertilizer use efficiency, stress tolerance and yield improvement of crops. However, because of the complexity of root traits and difficulty in phenotyping deep roots, the study on mechanisms of root development is rarely reported in wheat (Triticum aestivum L.). In this study, we identified that the LBD (LATERAL ORGAN BOUNDARIES DOMAIN) gene TaMOR (MORE ROOT in wheat) determines wheat crown root initiation. The mor mutants exhibited less or even no crown root, dwarfism, less grain number and lodging caused by few roots. The observation of cross sections showed that crown root initiation is inhibited in the mor mutants. Molecular assays revealed that TaMOR interacts with the auxin response factor ARF5 to directly induce the expression of the auxin transporter gene PIN2 (PIN-FORMED 2) in the root base to regulate crown root initiation. In addition, a 159-bp MITE (miniature inverted-repeat transposable element) insertion causing DNA methylation and lower expression of TaMOR-B was identified in TaMOR-B promoter, which is associated with lower root dry weight and shorter plant height. The results bring new light into regulation mechanisms of crown root initiation and offer a new target for the improvement of root system architecture in wheat.

中文翻译:

TaMOR 对小麦的根系启动和根系结构的改善至关重要

优化的根系结构有利于作物的水肥利用效率、抗逆性和产量提高。然而,由于根系性状的复杂性和深根表型的困难,小麦( Triticum aestivum L.)根系发育机制的研究鲜有报道。在这项研究中,我们发现 LBD(LATERAL ORGAN BOUNDARIES DOMAIN)基因TaMOR(小麦中的MORE ROOT)决定了小麦冠根的起始。mor突变体表现出较少甚至没有冠根、矮化、粒数少和根少引起的倒伏。横断面观察表明,在mor中,冠根萌生受到抑制。突变体。分子分析表明,TaMOR 与生长素反应因子 ARF5 相互作用,直接诱导生长素转运蛋白基因PIN2 ( PIN-FORMED 2 ) 在根基中的表达,从而调节冠根起始。此外,在TaMOR-B启动子中发现了一个 159-bp MITE(微型反向重复转座元件)插入,导致 DNA 甲基化和TaMOR-B表达降低,这与较低的根干重和较短的植物高度有关。该结果为研究冠根起始调控机制提供了新的思路,为小麦根系结构的改善提供了新的目标。
更新日期:2021-12-10
down
wechat
bug