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Karyotype and COI gene sequence of Chironomus heteropilicornis Wülker, 1996 (Diptera, Chironomidae) from the Gydan Peninsula, Russia
Comparative Cytogenetics ( IF 1 ) Pub Date : 2021-12-07 , DOI: 10.3897/compcytogen.v15i4.73135
Viktor V Bolshakov 1 , Alexander A Prokin 1, 2 , Sergey V Artemenko 3
Affiliation  

 The karyotype features and gene COI sequence of Chironomus heteropilicornis Wülker, 1996 from the Gydan Peninsula are presented for the first time. Nine banding sequences were determined, eight of them hpiA2, hpiB1, hpiC1, hpiC2, hpiD1, hpiE1, hpiF3 and hpiG1 were previously known from European, Georgian (South Caucasus) and Siberian populations. One new banding sequence for Ch. heteropilicornis, hpiB2, was found. The hpiA2 banding sequence was found in all individuals, and this is its second finding after the Georgian population (Karmokov 2019). The hpiF3 banding sequence was found only in the homozygous state. Additional B-chromosomes are absent. The genetic distances (K2P) between Ch. heteropilicornis COI gene sequence from Gydan Peninsula and Norway are 1.1­–1.3%, and Georgia – 1.8%, much lower than the commonly accepted threshold of 3% for species of genus Chironomus Meigen, 1803. The phylogenetic tree for COI gene sequences estimated by Bayesian inference showed geographically determined clusters of Norway and Gydan and a separate lineage of the Georgian population of Ch. heteropilicornis. The analysis of karyotype and COI gene sequences shows that the population of Ch. heteropilicornis from the Gydan Peninsula has an intermediate position within the Ch. pilicornis group between Georgian, Yakutian and Norwegian populations. The position of Ch. pilicornis Fabricius, 1787 from Canada and Greenland on the phylogenetic tree is discussed.

中文翻译:

1996 年俄罗斯吉丹半岛摇蚊(双翅目,摇蚊科)的核型和 COI 基因序列

 首次报道了1996年吉丹半岛异毛角摇蚊的核型特征和基因COI序列。确定了九个条带序列,其中八个 hpiA2、hpiB1、hpiC1、hpiC2、hpiD1、hpiE1、hpiF3 和 hpiG1 先前已知来自欧洲、格鲁吉亚(南高加索)和西伯利亚种群。Ch的一个新的条带序列。发现了heteropilicornis hpiB2。在所有个体中都发现了 hpiA2 条带序列,这是继格鲁吉亚种群之后的第二个发现(Karmokov 2019)。hpiF3 条带序列仅在纯合状态下被发现。不存在额外的 B 染色体。Ch之间的遗传距离(K2P)。来自Gydan半岛和挪威的heteropilicornis COI基因序列为1.1-1.3%,格鲁吉亚为1.8%,远低于通常接受的 Chironomus Meigen 属物种 3% 的阈值,1803 年。通过贝叶斯推断估计的 COI 基因序列的系统发育树显示了挪威和 Gydan 的地理确定集群以及 Ch. 的格鲁吉亚种群的单独谱系。异毛角兽。核型和COI基因序列分析表明,Ch的种群。来自Gydan半岛的heteropilicornis在Ch中具有中间位置。格鲁吉亚人、雅库特人和挪威人之间的 pilcornis 组。Ch的位置。pilcornis Fabricius,1787 年对来自加拿大和格陵兰的系统发育树进行了讨论。通过贝叶斯推断估计的 COI 基因序列的系统发育树显示了挪威和吉丹的地理确定集群以及 Ch. 的格鲁吉亚种群的独立谱系。异毛角兽。核型和COI基因序列分析表明,Ch的种群。来自Gydan半岛的heteropilicornis在Ch中具有中间位置。格鲁吉亚人、雅库特人和挪威人之间的 pilcornis 组。Ch的位置。pilcornis Fabricius,1787 年对来自加拿大和格陵兰的系统发育树进行了讨论。通过贝叶斯推断估计的 COI 基因序列的系统发育树显示了挪威和吉丹的地理确定集群以及 Ch. 的格鲁吉亚种群的独立谱系。异毛角兽。核型和COI基因序列分析表明,Ch的种群。来自Gydan半岛的heteropilicornis在Ch中具有中间位置。格鲁吉亚人、雅库特人和挪威人之间的 pilcornis 组。Ch的位置。pilcornis Fabricius,1787 年对来自加拿大和格陵兰的系统发育树进行了讨论。雅库特人和挪威人。Ch的位置。pilcornis Fabricius,1787 年对来自加拿大和格陵兰的系统发育树进行了讨论。雅库特人和挪威人。Ch的位置。pilcornis Fabricius,1787 年对来自加拿大和格陵兰的系统发育树进行了讨论。
更新日期:2021-12-07
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