A robust endoxylanase with high activity has great potential in biomass utilization. Here, the catalytic efficiency of the endoxylanase XynF derived from Herbinix hemicellulosilytica was improved by fusing a β-xylosidase xln-DT derived from Dictyoglomus thermophilum with linkers (L0 and L3) and the direction from xln-DT to XynF. The biochemical properties and application potential of chimeras towards corncob xylan were explored. The result showed that the chimeras Xln-L0-XynF and Xln-L3-XynF exhibited higher catalytic efficiency (31-fold and 20-fold enhancement, respectively), and maintained high stability in some harsh environments compared with parental enzymes. This might be attributed to the appropriate linkers and the direction which decrease the adverse interactions between the two modules. These two chimeras could hydrolyze corncob xylan to produce approximately 30% xylooligosaccharides with polymerization of 2–6. This work represents the promising xylanases for participating in XOS production from an abundant corncobs and promote us to modify glycoside hydrolase by fusion technology.

具有高活性的强健木聚糖内切酶在生物质利用方面具有巨大潜力。在这里，通过融合源自嗜热网纹藻的 β-木糖苷酶 xln-DT 提高了源自Herbinix hemicellulosilytica的木聚糖内切酶 XynF 的催化效率带有链接器（L0 和 L3）以及从 xln-DT 到 XynF 的方向。探讨了嵌合体对玉米芯木聚糖的生化特性和应用潜力。结果表明，与亲本酶相比，嵌合体 Xln-L0-XynF 和 Xln-L3-XynF 表现出更高的催化效率（分别提高 31 倍和 20 倍），并在一些恶劣的环境中保持高稳定性。这可能归因于适当的接头和减少两个模块之间不利相互作用的方向。这两种嵌合体可以水解玉米芯木聚糖，产生约 30% 的低聚木糖，聚合度为 2-6。这项工作代表了有前途的木聚糖酶参与从丰富的玉米芯中生产 XOS，并促进我们通过融合技术修饰糖苷水解酶。