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Sulfidic acetate mineralization at 45°C by an aquifer microbial community: key players and effects of heat changes on activity and community structure
Environmental Microbiology ( IF 5.1 ) Pub Date : 2021-12-02 , DOI: 10.1111/1462-2920.15852
Mohammad S Bin Hudari 1 , Carsten Vogt 1 , Hans H Richnow 1
Affiliation  

High-Temperature Aquifer Thermal Energy Storage (HT-ATES) is a sustainable approach for integrating thermal energy from various sources into complex energy systems. Temperatures ≥45°C, which are relevant in impact zones of HT-ATES systems, may dramatically influence the structure and activities of indigenous aquifer microbial communities. Here, we characterized an acetate-mineralizing, sulfate-reducing microbial community derived from an aquifer and adapted to 45°C. Acetate mineralization was strongly inhibited at temperatures ≤25°C and 60°C. Prolonged incubation at 12°C and 25°C resulted in acetate mineralization recovery after 40–80 days whereas acetate was not mineralized at 60°C within 100 days. Cultures pre-grown at 45°C and inhibited for 28 days by incubation at 12°C, 25°C, or 60°C recovered quickly after changing the temperature back to 45°C. Phylotypes affiliated to the order Spirochaetales and to endospore-forming sulfate reducers of the order Clostridiales were highly abundant in microcosms being active at 45°C highlighting their key role. In summary, prolonged incubation at 45°C resulted in active microbial communities mainly consisting of organisms adapted to temperatures between the typical temperature range of mesophiles and thermophiles and being resilient to temporary heat changes.

中文翻译:

含水层微生物群落在 45°C 的硫酸盐矿化:关键参与者和热量变化对活动和群落结构的影响

高温含水层热能储存 (HT-ATES) 是一种可持续的方法,用于将各种来源的热能整合到复杂的能源系统中。与 HT-ATES 系统的影响区相关的≥45°C 的温度可能会极大地影响本地含水层微生物群落的结构和活动。在这里,我们描述了一种源自含水层并适应 45°C 的醋酸盐矿化、硫酸盐还原微生物群落。乙酸盐矿化在 ≤25°C 和 60°C 的温度下受到强烈抑制。在 12°C 和 25°C 下长时间孵育导致醋酸盐矿化在 40-80 天后恢复,而醋酸盐在 60°C 下 100 天内没有矿化。培养物在 45°C 下预生长并通过在 12°C、25°C、或 60°C 在将温度改回 45°C 后迅速恢复。隶属于该命令的系统发育型螺旋体和梭菌目内生孢子形成硫酸盐还原剂在 45°C 下活跃的微观世界中非常丰富,突出了它们的关键作用。总之,在 45°C 下长时间孵育导致活跃的微生物群落主要由适应中温菌和嗜热菌典型温度范围之间温度的生物组成,并且对暂时的热变化具有弹性。
更新日期:2021-12-02
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