Molybdenum trioxide (MoO₃) as an artificial enzyme has attracted extensive attention owing to its striking merits. However, it still faces the challenges in poor catalytic activity or low production yield which could be addressed through rational structure design and mechanism elucidation. Herein, a glucose-functionalized oxygen vacancy-enriched molybdenum oxide (G-MoO_3-x) is prepared via an environmentally friendly, mass-production and low-temperature hydrothermal method. The G-MoO_3-x exhibits excellent bienzyme-like (oxidase and peroxidase) activities and outstanding photothermal conversion capacity (∼45.98%). Systematic studies reveal that oxygen vacancy is the determining factor for both enzyme-like activity and photothermal effect of G-MoO_3-x, and the peroxidase-like activity is positively correlated with oxygen vacancy concentration. Density functional theory (DFT) calculations indicate that the oxygen (O)-defect MoO₃ has a more favorable impact on peroxidase-like activity than MoO₃ edge. Through all-in-one synergistic effects, G-MoO_3-x nanozyme exhibits significant broad-spectrum antibacterial properties against β-lactamase-producing Escherichia coli and methicillin-resistant Staphylococcus aureus as well as its biofilm.

三氧化钼（MoO ₃）作为一种人工酶因其显着的优点而受到广泛关注。然而，它仍然面临催化活性差或产率低的挑战，可以通过合理的结构设计和机理阐明来解决。在此，通过环境友好、大规模生产和低温水热法制备了葡萄糖功能化的富氧空位氧化钼（G-MoO _3-x）。G-MoO _3-x表现出优异的类双酶（氧化酶和过氧化物酶）活性和出色的光热转换能力（~45.98%）。系统研究表明氧空位是 G-MoO 类酶活性和光热效应的决定因素_3-x，并且过氧化物酶样活性与氧空位浓度呈正相关。密度泛函理论 (DFT) 计算表明，氧 (O) 缺陷 MoO ₃对过氧化物酶样活性的影响比 MoO ₃边缘更有利。通过多合一的协同作用，G-MoO _3-x纳米酶对产β-内酰胺酶的大肠杆菌和耐甲氧西林的金黄色葡萄球菌及其生物膜表现出显着的广谱抗菌特性。