Field evaluation of cotton for resistance to Fusarium oxysporum f. sp. vasinfectum Race 4 (FOV4) is important in breeding. In this study, an 8 × 8 Latin square design was arranged in two FOV4-infested fields each with two trials to evaluate eight cotton (Gossypium hirstum) cultivars based on mortality rate (MR) and root vascular staining (RVS). For a comparison, two trials of the same design were grown in the greenhouse. Consistent with the mapping pattern of MR in the Texas field, the analysis of variance confirmed the existence of effects due to tiers not planting rows in the Texas location only. Significant variation due to cultivars was consistently detected in the two trials of both locations. The repeatability was confirmed by significant correlation coefficients between most tiers (replications) and between the two trials in each field. The greenhouse test had the highest mean MR (72–78%) and lowest coefficients of variation (CV; 17–28%), followed by the Texas field with a lower MR (36–57%) and similar CV (22–29%); and the New Mexico test had the highest CV (59–73%) and the lowest MR (13–18%). The MR between the two fields and between the fields and the greenhouse were significantly correlated. ‘Pima S-7’ had the highest MR (79–93%), whereas ‘FM 2334GLT’ had the lowest (15–35%), consistent with RVS at crop maturity. Among randomized complete block designs (RCBD) with three to seven replications, the mean squared error and CV were the lowest in RCBD with three replications.

中文翻译：

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使用拉丁方设计来评估棉花抗枯萎病第 4 种田间评价中的实验误差

棉花对尖孢镰刀菌的抗性田间评价。sp。vasinfectum Race 4 (FOV4) 在育种中很重要。在这项研究中，一个 8 × 8 的拉丁方设计被安排在两个 FOV4 出没的田地中，每个田地有两个试验来评估八种棉花（Gossypium hirstum) 基于死亡率 (MR) 和根血管染色 (RVS) 的品种。为了进行比较，在温室中进行了两个相同设计的试验。与德克萨斯州田间的 MR 映射模式一致，方差分析证实了由于层级仅在德克萨斯州位置不种植行而存在的影响。在两个地点的两次试验中始终检测到因品种引起的显着变化。大多数层（重复）之间以及每个领域的两个试验之间的显着相关系数证实了可重复性。温室试验的平均 MR 最高 (72-78%) 和变异系数最低 (CV; 17-28%)，其次是德克萨斯油田，MR 较低 (36-57%) 和相似的 CV (22-29 %); 新墨西哥测试的 CV 最高（59-73%），MR 最低（13-18%）。两块田间以及田间与温室之间的MR显着相关。'Pima S-7' 的 MR 最高 (79–93%)，而 'FM 2334GLT' 最低 (15–35%)，与作物成熟时的 RVS 一致。在具有 3 到 7 次重复的随机完整区组设计 (RCBD) 中，均方误差和 CV 在具有 3 次重复的 RCBD 中最低。