Abstract

In this present study, marine red algae Hypnea valentiae capped silver nanoparticles (Hv-AgNPs) have been prepared through the green method and characterized by various analytical tools. The prepared Hv-AgNPs were confirmed by Ultraviolet-visible (UV) spectroscopy at 430 nm. The X-ray diffraction (XRD) analysis affirmed the face-centered cubic crystalline structure of Hv-AgNPs. Transmission electron microscope (TEM) revealed the Hv-AgNPs shape as spherical with an average size of 10–45 nm. Fourier Transform Infrared (FR-IR) analysis depicted the presence of various biomolecules such as carboxyl, phenolic, and amine groups, which acted as reducing and capping agents of biosynthesized silver nanoparticles. The Hv-AgNPs had significant antibacterial activity against four bacterial pathogens. The 2,2’-Diphenyl-1-picrylhydrazy (DPPH) assay revealed the strong free radical scavenging ability of Hv-AgNPs. The Hv-AgNPs exhibited excellent anti-cancer activity against HT-29 human colon cancer (IC50–24.6 μg/ml) and A549 lung cancer cell lines (IC50–5.917 µg/ml) by MTT assay. Furthermore, the investigations demonstrated that green synthesized silver nanoparticles possess high anti-bacterial, anti-oxidant and anti-cancer activities.

摘要

在本研究中，海洋红藻Hypnea valentiae已通过绿色方法制备了封端银纳米颗粒（Hv-AgNPs），并通过各种分析工具对其进行了表征。制备的 Hv-AgNPs 通过 430 nm 的紫外-可见 (UV) 光谱进行确认。X射线衍射（XRD）分析证实了Hv-AgNPs的面心立方晶体结构。透射电子显微镜 (TEM) 显示 Hv-AgNPs 形状为球形，平均尺寸为 10-45 nm。傅里叶变换红外 (FR-IR) 分析描述了各种生物分子的存在，例如羧基、酚和胺基团，它们充当生物合成银纳米粒子的还原剂和封端剂。Hv-AgNPs对四种细菌病原体具有显着的抗菌活性。2,2'-Diphenyl-1-picrylhydrazy (DPPH) 测定揭示了 Hv-AgNPs 的强自由基清除能力。通过 MTT 测定，Hv-AgNPs 对 HT-29 人结肠癌 (IC50–24.6 μg/ml) 和 A549 肺癌细胞系 (IC50–5.917 μg/ml) 表现出优异的抗癌活性。此外，研究表明，绿色合成的银纳米粒子具有很高的抗菌、抗氧化和抗癌活性。