β-d-fructofuranosyl glycosidases are enzymes that produce health-beneficial fructooligosaccharides from natural fructans. In a recent issue of JBC, Kashima et al. identified a novel α-d-fructofuranosyl-active enzyme, αFFase1, from the caries-associated bacterium Bifidobacterium dentium. αFFase1 reversibly forms a potential prebiotic also found in caramel, difructose dianhydride I, via intramolecular condensation of the substrate inulobiose. Kashima et al. elegantly combine NMR, X-ray crystallography, and molecular dynamics to describe an original mechanism for the reversible reactions catalyzed by αFFase1 that establishes the new glycoside hydrolase family GH172.

中文翻译：

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一种健康的双歧杆菌焦糖鸡尾酒。

β-d-呋喃果糖苷酶是从天然果聚糖中产生对健康有益的低聚果糖的酶。在最近一期 JBC 中，Kashima 等人。从龋齿相关细菌双歧杆菌中鉴定出一种新的 α-d-呋喃果糖基活性酶 αFFase1。αFFase1 通过底物菊糖二糖的分子内缩合可逆地形成一种潜在的益生元，这种益生元也存在于焦糖二果糖二酐 I 中。鹿岛等人。将 NMR、X 射线晶体学和分子动力学巧妙地结合起来，描述了由 αFFase1 催化的可逆反应的原始机制，该机制建立了新的糖苷水解酶家族 GH172。