In situ-based studies on microbiome-host interactions after arsenic exposure are few. In this study, the variations in arsenics, microbiota, and host genes along murine intestinal tracts were determined after arsenic exposure for two months. There was a gradual increase in the concentration of total As (C_tAs) in feces from ileum to colon, whereas C_tAs in the corresponding tissues were relatively stable. Differences in arsenic levels between feces and tissues were significantly different. The proportion of arsenite (iAs^Ⅲ) in feces gradually decreased, however, it gradually increased in tissues. After arsenic exposure, the diversity and abundance of microbial community and networks in each segment were significantly dysregulated. Notably, 328, 579 and 90 differently expressed genes were detected in ileum, cecum, and colon, respectively. In addition, microbiome and transcriptome analyses showed a significant correlation between the abundance of Faecalibaculum and expressions of Plb1, Hspa1b, Areg and Duoxa2 genes. This implies that they may be involved in arsenic biotransformation. In vitro experiments using Biofidobactrium and Lactobacillus showed that probiotics have arsenic transformation abilities. Therefore, gut microbiome may modulate arsenic accumulation, excretion and detoxification along the digestive tract. Moreover, the abundance and diversity of gut microbiome may be related to the changes in host health.

关于砷暴露后微生物组与宿主相互作用的原位研究很少。在这项研究中，在砷暴露两个月后，确定了小鼠肠道中砷、微生物群和宿主基因的变化。从回肠到结肠的粪便中总As( C _{tAs )浓度逐渐升高，而相应组织中的}C _tAs相对稳定。粪便和组织间砷含量的差异有显着差异。亚砷酸盐（iAs ^Ⅲ）在粪便中逐渐减少，但在组织中逐渐增加。砷暴露后，各段微生物群落和网络的多样性和丰度显着失调。值得注意的是，分别在回肠、盲肠和结肠中检测到 328、579 和 90 个不同表达的基因。此外，微生物组和转录组分析显示粪杆菌的丰度与Plb1 、 Hspa1b 、 Areg和Duoxa2基因的表达之间存在显着相关性。这意味着它们可能参与了砷的生物转化。使用Biofidobactrium和Lactobacillus的体外实验表明益生菌具有砷转化能力。因此，肠道微生物组可以调节砷沿消化道的积累、排泄和解毒。此外，肠道微生物组的丰度和多样性可能与宿主健康的变化有关。