The previous study has shown that transcriptional factor MEOX1 could promote proliferation and sphere formation ability of non-small cell lung cancer (NSCLC) cells, however, we found that MEOX1 mRNA was lowly expressed in lung cancer tissues compared to that in normal adjacent tissues, and MEOX1 mRNA expression was positively correlated with the survival of lung cancer patients, especially in lung adenocarcinoma patients. Functional experiments using in vitro and in vivo experiments revealed that stable overexpression of MEOX1 significantly suppressed the proliferation ability, promoted cell cycle arrest in G2 phase, and apoptotic ability of NSCLC cells. Additionally, it was identified that MEOX1 and CCNB1 mRNA expression exhibited a negative correlation in different lung cancer tissues. Mechanistically, we indicated that MEOX1 bound to the transcriptional initiation site of CCNB1 and thus suppressed CCNB1 expression. Notably, CCNB1 overexpression rescued the inhibition of MEOX1 overexpression on NSCLC progression. This study deciphers a novel MEOX1/CCNB1 axis suppressing NSCLC progression.

中文翻译：

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MEOX1通过抑制细胞周期检查点基因CCNB1来抑制肺癌细胞的进展

先前的研究表明，转录因子 MEOX1 可以促进非小细胞肺癌 (NSCLC) 细胞的增殖和球体形成能力，然而，我们发现MEOX1 mRNA 在肺癌组织中的表达低于正常癌旁组织，并且MEOX1 mRNA 的表达与肺癌患者的生存率呈正相关，尤其是在肺腺癌患者中。使用体外和体内实验进行的功能实验表明，MEOX1的稳定过表达显着抑制了 NSCLC 细胞的增殖能力，促进了 G2 期细胞周期停滞和凋亡能力。此外，还发现MEOX1和CCNB1mRNA表达在不同肺癌组织中呈负相关。从机制上讲，我们表明 MEOX1 与 CCNB1 的转录起始位点结合，从而抑制了 CCNB1的表达。值得注意的是，CCNB1过表达挽救了MEOX1过表达对 NSCLC 进展的抑制作用。本研究破译了一种新的 MEOX1/CCNB1 轴抑制 NSCLC 进展。