This study examined the physiological responses of the larval stages of Haliotis tuberculata, an economically important abalone, to combined temperature (17 °C and 19 °C) and pH (ambient pH and −0.3 units, i.e., +200% increase in seawater acidity) in a full factorial experiment. Tissue organogenesis, shell formation, and shell length significantly declined due to low pH. High temperature significantly increased the proportion of fully shelled larvae at 24 h post-fertilization (hpf), but increased the proportion of unshelled larvae at 72 hpf. Percentage of swimming larvae at 24 hpf, 72 hpf and 96 hpf significantly declined due to high temperature, but not because of low pH. Larval settlement increased under high temperature, but was not affected by low pH. Despite the fact that no interaction between temperature and pH was observed, the results provide additional evidence on the sensitivity of abalone larvae to both low pH and high temperature. This may have negative consequences for the persistence of abalone populations in natural and aquaculture environments in the near future.

本研究检测了蝾螈幼虫阶段的生理反应，一种经济上重要的鲍鱼，在全因子实验中结合温度（17°C 和 19°C）和 pH（环境 pH 和 -0.3 单位，即海水酸度增加 +200%）。由于低 pH 值，组织器官发生、壳形成和壳长度显着下降。高温显着增加了受精后24小时（hpf）的全壳幼虫比例，但在72 hpf时增加了无壳幼虫的比例。由于高温，24 hpf、72 hpf 和 96 hpf 的游泳幼虫百分比显着下降，但不是因为低 pH 值。高温下幼虫沉降增加，但不受低pH值的影响。尽管没有观察到温度和pH之间的相互作用，这些结果为鲍鱼幼虫对低 pH 值和高温的敏感性提供了额外的证据。这可能在不久的将来对自然和水产养殖环境中鲍鱼种群的持续存在产生负面影响。