Reverse osmosis membrane is widely applied in advanced wastewater treatment. Along with the production of clean water, 30%∼50% reverse osmosis concentrated water (W_ROC), containing massive nitrogen and phosphorus, is also produced. W_ROC would bring ecological risk if discharged into the natural water body. Hence, attached microalgae cultivation based on W_ROC was proposed to recovery the nutrients from water in this study. The results showed that the initial concentrations of nitrogen and phosphorus in W_ROC (TN≈50 mg/L, TP≈10 mg/L) were not too high to inhibit attached microalgae growth. Among the complex chemicals in W_ROC, non-oxidizing bacteriostatic agent had the worst impact on the growth of microalgae with an 8-day inhibition ratio of 25%. Compared with the microalgae cultured by nutrient medium, the lipid and protein contents in attached microalgae cultured by W_ROC were relatively high and stable during the whole cultivation period. Seen from the micro level, there was no obvious resistance to the transfer of TN and TP in the microalgae biofilm. However, the photosynthetic rate of microalgae in the biofilm decreases along with the depth. In conclusion, uneven light distribution in biofilm and the non-oxidizing bacteriostatic agent in W_ROC were the main resistance to the growth of attached cultured microalgae, which should be optimized or per-treated.

反渗透膜广泛应用于污水深度处理。随着纯净水的生产，还产生了30%～50%的反渗透浓缩水（W _ROC），其中含有大量的氮和磷。W _ROC排放到天然水体中会带来生态风险。因此，本研究提出了基于 W _{ROC 的}附着微藻培养以从水中回收养分。结果表明，W _ROC中氮和磷的初始浓度（TN≈50 mg/L，TP≈10 mg/L）并不太高，无法抑制附着微藻的生长。在 W _ROC的复杂化学品中,非氧化性抑菌剂对微藻生长的影响最差，8天抑制率为25%。与营养培养基培养的微藻相比，W _ROC培养的贴壁微藻在整个培养期内脂质和蛋白质含量较高且稳定。从微观层面看，微藻生物膜对TN和TP的转移没有明显的阻力。然而，生物膜中微藻的光合速率随着深度的增加而降低。综上所述，生物膜光分布不均和W _ROC中的非氧化性抑菌剂是附着培养微藻生长的主要阻力，应优化或过处理。