Understanding disease-related processes at the molecular level is of great importance for the prevention and treatment of diseases. However, due to the lack of effective analytical tools, it is challenging to gain insight into the relationships between a specific bioactive molecule and the associated disease. Herein, a rapid turn-on resorufin-based fluorescent probe platform utilizing the HClO-specific oxidative cleavage of the amide was constructed, allowing the visualization of HClO in vitro and in vivo. These probes could quickly respond to HClO (< 50 s) with high selectivity and sensitivity (12–153 nM). The probe REClO-6 had the fastest response (30 s) and the highest sensitivity (12 nM), and was successfully used for the imaging of endogenous and exogenous HClO in cells and zebrafish. Notably, it was also successfully applied to the imaging of HClO in mouse arthritis and solid tumors. This study provided a rapid imaging analysis tool, which would be used to investigate the relationship between HClO and the disease-related physiological processes.

从分子水平了解疾病相关过程对于疾病的预防和治疗具有重要意义。然而，由于缺乏有效的分析工具，很难深入了解特定生物活性分子与相关疾病之间的关系。在此，构建了一个快速开启的基于试卤灵的荧光探针平台，该平台利用 HClO 对酰胺的特异性氧化裂解，实现了 HClO 在体外和体内的可视化。 这些探针可以以高选择性和灵敏度 (12–153  nM)快速响应 HClO (< 50 s)。探头REClO-6具有最快的响应（30  s）和最高的灵敏度（12 nM)，并成功用于细胞和斑马鱼中内源性和外源性 HClO 的成像。值得注意的是，它还成功地应用于 HClO 在小鼠关节炎和实体瘤中的成像。本研究提供了一种快速成像分析工具，可用于研究 HClO 与疾病相关生理过程之间的关系。