Long-term anaerobic digestion of switchgrass (SG) was investigated in batch and semi-continuous reactors operating at different Substrate to Inoculum (S:I) ratios and organic loading rates (OLRs) by monitoring process stability, microbial community structure, and investigating kinetic modeling. The highest methane yield of 204 mLCH4/gVS was observed when the batch system was operated at the S:I ratio of 1.1. Methane yields in the semi-continuous configuration were around 148, 157, and 60 mLCH4/gVS at OLRs of 0.75, 1.0, and 1.5 gVS/(L.d), respectively. A drastic decrease in methane yields was observed at the highest OLR value of 1.5 gVS/(L.d). The optimum OLR in the semi-continuous configuration was decided as 1.0 gVS/(L.d) where 35% of the SG theoretical methane yield and 38% energy recovery were attained. Several methanogenic archaea, Methanosarcina, Methanosaeta, Methanothrix, and Methanomethylovorans, together with bacteria species, Chloroflexi, Firmicutes, Spirochaetes, and Actinobacteria were observed in the semi-continuous configuration after 270 days of operation. The dominant bacterial phyla of s-CSTR were mostly members of Spirochaetes phylum, which plays a considerable role in acetate oxidation under either mesophilic or thermophilic conditions.

通过监测过程稳定性、微生物群落结构和研究动力学，在不同底物与接种物 (S:I) 比率和有机负载率 (OLR) 下运行的分批和半连续反应器中研究柳枝稷 (SG) 的长期厌氧消化造型。当分批系统以 1.1 的 S:I 比操作时，观察到最高的甲烷产量为 204 mLCH4/gVS。半连续配置中的甲烷产量分别为 148、157 和 60 mLCH4/gVS，OLR 分别为 0.75、1.0 和 1.5 gVS/(Ld)。在 1.5 gVS/(Ld) 的最高 OLR 值下观察到甲烷产量急剧下降。半连续配置中的最佳 OLR 确定为 1.0 gVS/(Ld)，其中 SG 理论甲烷产率的 35% 和能量回收率达到 38%。几种产甲烷古菌，绿弯菌门，厚壁菌门，螺旋体，和放线菌在半连续配置，观察手术后270天。s-CSTR 的优势细菌门主要是螺旋体门的成员，在中温或高温条件下，它在乙酸盐氧化中起着相当大的作用。