Enzymatic hydrolysis of waste biomass for bioethanol production is considered a decades old traditional, inexpensive, and energy-effective approach. In this study, waste office paper was pretreated with diluted sulfuric acid (H₂SO₄) and hydrolyzed with one of the most available and cost-effective enzymes, cellulase derived from Trichoderma reesei, under submerged static condition. Three different pretreatment approaches—cut into 2 cm², blended with distilled water, and pretreated with diluted H2SO4—have been implemented, and pretreatment with diluted H₂SO₄ was the most effective. Hydrolysis with different concentrations—0.5 M, 1.0 M, 1.5 M, 2.0 M of H₂SO₄—was performed. The maximum glucose content was obtained at 2.0 M H₂SO₄ at 90 min reaction time, and glucose yield was 0.11 g glucose/g wastepaper. The cut paper, wet-blended, and acid-treated wastepaper was hydrolyzed with cellulase enzyme for 2, 4, and 5 consecutive days with 5 mg, 10 mg, 15 mg, and 20 mg enzyme loadings. The maximum glucose content obtained was 9.75 g/l from acid-treated wastepaper, after 5 days of enzymatic hydrolysis with 20 mg enzyme loading and a glucose yield of a 0.5 g glucose/g wastepaper. The wastepaper hydrolysate was further fermented for 6, 8, and 10 hours continuously with Saccharomyces cerevisiae (yeast), and at 10 hours of fermentation, the maximum glucose consumption was 0.18 g by yeast. Further, HPLC analysis of the fermented medium presented a strong peak of bioethanol content at 16.12 min. The distillation of bioethanol by rotary evaporator presented 0.79 ml bioethanol/fermented solution, which indicated the conversion efficiency of 79%.

用于生物乙醇生产的废弃生物质的酶解被认为是一种已有数十年历史的传统、廉价且节能的方法。在这项研究中，废办公纸用稀硫酸 (H ₂ SO ₄ )进行预处理，并在浸没静态条件下用一种最容易获得且最具成本效益的酶，即来自里氏木霉的纤维素酶进行水解。已经实施了三种不同的预处理方法——切成 2 cm ²，与蒸馏水混合，用稀 H2SO4 预处理，其中用稀 H ₂ SO ₄预处理是最有效的。不同浓度的水解——0.5 M、1.0 M、1.5 M、2.0 M的H ₂ SO_4个-was进行。在反应时间为 2.0 MH ₂ SO ₄时获得最大葡萄糖含量，反应时间为 90 分钟，葡萄糖产量为 0.11 g 葡萄糖/g 废纸。用纤维素酶连续 2、4 和 5 天以 5 毫克、10 毫克、15 毫克和 20 毫克的酶加载量对切纸、湿混和酸处理的废纸进行水解。在用 20 mg 酶负载和 0.5 g 葡萄糖/g 废纸的葡萄糖产率进行 5 天的酶水解后，从酸处理的废纸中获得的最大葡萄糖含量为 9.75 g/l。将废纸水解物与酿酒酵母连续发酵6、8、10小时（酵母），在发酵 10 小时时，酵母的最大葡萄糖消耗量为 0.18 克。此外，发酵培养基的 HPLC 分析在 16.12 分钟处呈现出生物乙醇含量的强峰。旋转蒸发仪蒸馏生物乙醇得到0.79ml生物乙醇/发酵液，转化率为79%。