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Renaissance for Phage-Based Bacterial Control
Environmental Science & Technology ( IF 11.4 ) Pub Date : 2021-11-18 , DOI: 10.1021/acs.est.1c06232
Cory Schwarz 1, 2 , Jacques Mathieu 1, 2 , Jenny A Laverde Gomez 1, 2 , Pingfeng Yu 1 , Pedro J J Alvarez 1, 2
Affiliation  

Bacteriophages (phages) are an underutilized biological resource with vast potential for pathogen control and microbiome editing. Phage research and commercialization have increased rapidly in biomedical and agricultural industries, but adoption has been limited elsewhere. Nevertheless, converging advances in DNA sequencing, bioinformatics, microbial ecology, and synthetic biology are now poised to broaden phage applications beyond pathogen control toward the manipulation of microbial communities for defined functional improvements. Enhancements in sequencing combined with network analysis make it now feasible to identify and disrupt microbial associations to elicit desirable shifts in community structure or function, indirectly modulate species abundance, and target hub or keystone species to achieve broad functional shifts. Sequencing and bioinformatic advancements are also facilitating the use of temperate phages for safe gene delivery applications. Finally, integration of synthetic biology stands to create novel phage chassis and modular genetic components. While some fundamental, regulatory, and commercialization barriers to widespread phage use remain, many major challenges that have impeded the field now have workable solutions. Thus, a new dawn for phage-based (chemical-free) precise biocontrol and microbiome editing is on the horizon to enhance, suppress, or modulate microbial activities important for public health, food security, and more sustainable energy production and water reuse.

中文翻译:

基于噬菌体的细菌控制复兴

噬菌体(噬菌体)是一种未被充分利用的生物资源,具有巨大的病原体控制和微生物组编辑潜力。噬菌体研究和商业化在生物医学和农业行业迅速增加,但在其他地方的采用受到限制。然而,DNA 测序、生物信息学、微生物生态学和合成生物学方面的融合进展现在正准备将噬菌体的应用范围从病原体控制扩展到操纵微生物群落以实现明确的功能改进。测序的增强与网络分析相结合,现在可以识别和破坏微生物关联,从而引发群落结构或功能的理想转变,间接调节物种丰度,并瞄准枢纽或关键物种以实现广泛的功能转变。测序和生物信息学的进步也促进了温带噬菌体在安全基因传递应用中的应用。最后,合成生物学的整合有助于创造新的噬菌体底盘和模块化遗传成分。虽然广泛使用噬菌体的一些基本、监管和商业化障碍仍然存在,但许多阻碍该领域的主要挑战现在都有可行的解决方案。因此,基于噬菌体(无化学物质)的精确生物控制和微生物组编辑的新曙光即将出现,以增强、抑制或调节对公共卫生、粮食安全以及更可持续的能源生产和水再利用很重要的微生物活动。合成生物学的整合有助于创造新的噬菌体底盘和模块化遗传成分。虽然广泛使用噬菌体的一些基本、监管和商业化障碍仍然存在,但许多阻碍该领域的主要挑战现在都有可行的解决方案。因此,基于噬菌体(无化学物质)的精确生物控制和微生物组编辑的新曙光即将出现,以增强、抑制或调节对公共卫生、粮食安全以及更可持续的能源生产和水再利用很重要的微生物活动。合成生物学的整合有助于创造新的噬菌体底盘和模块化遗传成分。虽然广泛使用噬菌体的一些基本、监管和商业化障碍仍然存在,但许多阻碍该领域的主要挑战现在都有可行的解决方案。因此,基于噬菌体(无化学物质)的精确生物控制和微生物组编辑的新曙光即将出现,以增强、抑制或调节对公共卫生、粮食安全以及更可持续的能源生产和水再利用很重要的微生物活动。
更新日期:2021-11-18
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