The use, in veterinary practice, of a three-dimensional biopolymer matrix (based on chitosan, agarose, and gelatin and doped with halloysite nanotubes) as a vehicle for mesenchymal stem cells (MSCs) to repair bone defects is reported here. The nanocomposite, combined with allogenic adipose-derived stem cells, was suitable for the repair of bone defects in dogs when paired with standard surgery involving metal Kirshner wires. The absence of inflammatory reactions to biopolymer matrices with allogenic stem cells was revealed in the case of an animal prone to inflammatory and allergic reactions. In addition, positive dynamics in the fusion of chronic bone defects without rejection reactions was observed after using a biopolymer matrix with MSCs.

本文报道了在兽医实践中使用三维生物聚合物基质（基于壳聚糖、琼脂糖和明胶并掺杂埃洛石纳米管）作为间充质干细胞 (MSC) 修复骨缺损的载体。这种纳米复合材料与同种异体脂肪干细胞相结合，与涉及金属克什纳线的标准手术相结合，适用于修复狗的骨缺损。在易于发生炎症和过敏反应的动物的情况下，揭示了对具有同种异体干细胞的生物聚合物基质不存在炎症反应。此外，在将生物聚合物基质与 MSC 一起使用后，观察到慢性骨缺损融合的积极动态，而没有排斥反应。