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Heat stress-induced mucosal barrier dysfunction is potentially associated with gut microbiota dysbiosis in pigs
Animal Nutrition ( IF 6.3 ) Pub Date : 2021-11-16 , DOI: 10.1016/j.aninu.2021.05.012
Bing Xia 1 , Weida Wu 2 , Wei Fang 1, 3 , Xiaobin Wen 1 , Jingjing Xie 1 , Hongfu Zhang 1
Affiliation  

Heat stress (HS) can be detrimental to the gut health of swine. Many negative outcomes induced by HS are increasingly recognized as including modulation of intestinal microbiota. In turn, the intestinal microbiota is a unique ecosystem playing a critical role in mediating the host stress response. Therefore, we aimed to characterize gut microbiota of pigs’ exposure to short-term HS, to explore a possible link between the intestinal microbiota and HS-related changes, including serum cytokines, oxidation status, and intestinal epithelial barrier function. Our findings showed that HS led to intestinal morphological and integrity changes (villus height, serum diamine oxidase [DAO], serum D-lactate and the relative expressions of tight junction proteins), reduction of serum cytokines (interleukin [IL]-8, IL-12, interferon-gamma [IFN-γ]), and antioxidant activity (higher glutathione [GSH] and malondialdehyde [MDA] content, and lower superoxide dismutase [SOD]). Also, 16S rRNA sequencing analysis revealed that although there was no difference in microbial α-diversity, some HS-associated composition differences were revealed in the ileum and cecum, which partly led to an imbalance in the production of short-chain fatty acids including propionate acid and valerate acid. Relevance networks revealed that HS-derived changes in bacterial genera and microbial metabolites, such as Chlamydia, Lactobacillus, Succinivibrio, Bifidobacterium, Lachnoclostridium, and propionic acid, were correlated with oxidative stress, intestinal barrier dysfunction, and inflammation in pigs. Collectively, our observations suggest that intestinal damage induced by HS is probably partly related to the gut microbiota dysbiosis, though the underlying mechanism remains to be fully elucidated.



中文翻译:

热应激诱导的黏膜屏障功能障碍可能与猪肠道菌群失调有关

热应激 (HS) 可能对猪的肠道健康有害。HS 引起的许多负面结果越来越多地被认为包括肠道微生物群的调节。反过来,肠道微生物群是一个独特的生态系统,在介导宿主应激反应中发挥着关键作用。因此,我们旨在表征猪短期接触 HS 的肠道微生物群,以探索肠道微生物群与 HS 相关变化之间的可能联系,包括血清细胞因子、氧化状态和肠上皮屏障功能。我们的研究结果表明,HS 导致肠道形态和完整性的变化(绒毛高度、血清二胺氧化酶 [DAO]、血清 D-乳酸和紧密连接蛋白的相对表达)、血清细胞因子(白介素 [IL]-8、IL -12,干扰素-γ [IFN-γ]),和抗氧化活性(更高的谷胱甘肽 [GSH] 和丙二醛 [MDA] 含量,以及更低的超氧化物歧化酶 [SOD])。此外,16S rRNA 测序分析表明,尽管微生物 α 多样性没有差异,但回肠和盲肠中发现了一些与 HS 相关的组成差异,这部分导致了包括丙酸盐在内的短链脂肪酸的产生不平衡。酸和戊酸。相关网络显示,HS 衍生的细菌属和微生物代谢物的变化,例如 这部分导致了包括丙酸和戊酸在内的短链脂肪酸的生产不平衡。相关网络显示,HS 衍生的细菌属和微生物代谢物的变化,例如 这部分导致了包括丙酸和戊酸在内的短链脂肪酸的生产不平衡。相关网络显示,HS 衍生的细菌属和微生物代谢物的变化,例如衣原体、乳酸杆菌、琥珀弧菌、双歧杆菌、毛梭菌和丙酸与猪的氧化应激、肠道屏障功能障碍和炎症相关。总的来说,我们的观察表明,HS 引起的肠道损伤可能部分与肠道菌群失调有关,但其潜在机制仍有待充分阐明。

更新日期:2021-12-23
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