Background

Cordyceps militaris is a traditional medicinal mushroom containing some prominent bioactive compounds. This study aimed to investigate the effects of various grain substrates and cultivation patterns on the production of fruiting bodies, adenosine, cordycepin, and pentostatin of C. militaris by solid-state fermentation.

Methods

Seven grain substrates were prepared and inoculated with inoculum of C. militaris, then grown under four cultivation patterns. During the cultivation, the harvested samples were divided into fermented grains and fruiting bodies to dry, ground, extract, and determine the contents of bioactive compounds by high-performance liquid chromatography.

Significant Findings

The results showed that oats and Pattern I cultivation favored the fruiting body production. The content of adenosine and pentostatin in harvested fruiting bodies, irrespective of the grain type and cultivation patterns, was significantly higher than in the fermented grains. A higher content of adenosine and pentostatin in the fruiting bodies was observed for brown rice and buckwheat under Pattern I. Additionally, the coix seeds and the cultivation of control pattern favored the higher cordycepin production and revealed that the temperature treatment during the cultivation could increase the cordycepin content in fermented grains and fruiting bodies of C. militaris.

中文翻译：

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利用谷物基质和栽培模式提高蛹虫草子实体和生物活性化合物的产量

背景

蛹虫草是一种传统的药用蘑菇，含有一些突出的生物活性化合物。本研究旨在探讨不同谷物基质和栽培模式对固态发酵生产蛹虫草子实体、腺苷、虫草素和喷司他丁的影响。

方法

制备了七种谷物基质并接种了蛹虫草接种物，然后在四种栽培模式下生长。在栽培过程中，将收获的样品分为发酵粮和子实体进行干燥、研磨、提取，并通过高效液相色谱法测定生物活性化合物的含量。

重要发现

结果表明，燕麦和Ⅰ型栽培有利于子实体的产生。收获的子实体中腺苷和喷司他丁的含量，无论谷物类型和栽培方式如何，均显着高于发酵谷物。在模式 I 下观察到糙米和荞麦子实体中腺苷和喷司他丁的含量较高。此外，薏苡仁和对照模式的栽培有利于更高的虫草素产量，表明栽培过程中的温度处理可以增加虫草素的产量。蛹虫草发酵谷物和子实体中的虫草素含量。