In life science, rapid mutation detection in oligonucleotides is in a great demand for genomic and medical screening. To satisfy this demand, surface-enhanced resonance Raman spectroscopy (SERRS) in the deep-UV (DUV) regime offers a promising solution due to its merits of label-free nature, strong electromagnetic confinement, and charge transfer effect. Here, we demonstrate an epitaxial aluminum (Al) DUV-SERRS substrate that resonates effectively with the incident Raman laser and the ss-DNA at 266 nm, yielding significant SERRS signals of the detected analytes. For the first time, to the best of our knowledge, we obtaine SERRS spectra for all bases of oligonucleotides, not only revealing maximum characteristic Raman peaks but also recording the highest enhancement factor of up to 10⁶ for a 1 nm thick adenine monomer. Moreover, our epitaxial Al DUV-SERRS substrate is able to enhance the Raman signal of all four bases of 12-mer ss-DNA and to further linearly quantify the single-base mutation in the 12-mer ss-DNA.

中文翻译：

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演示卓越的深紫外表面增强共振拉曼散射 (SERRS) 底物和寡核苷酸中的单碱基突变检测

在生命科学中，寡核苷酸中的快速突变检测对基因组和医学筛查有很大的需求。为了满足这一需求，深紫外（DUV）区域的表面增强共振拉曼光谱（SERRS）由于其无标记性、强电磁限制和电荷转移效应等优点，提供了一种很有前景的解决方案。在这里，我们展示了一种外延铝 (Al) DUV-SERRS 基板，该基板可与入射拉曼激光和 266 nm 的 ss-DNA 有效共振，从而产生检测到的分析物的显着 SERRS 信号。据我们所知，我们第一次获得了寡核苷酸所有碱基的 SERRS 光谱，不仅揭示了最大特征拉曼峰，而且记录了高达 10 ^{6的最高增强因子}对于 1 nm 厚的腺嘌呤单体。此外，我们的外延 Al DUV-SERRS 基板能够增强 12-mer ss-DNA 的所有四个碱基的拉曼信号，并进一步线性量化 12-mer ss-DNA 中的单碱基突变。