The present study was intended to evaluate the effect of poly herbal formulation (PHF) made with three nutraceuticals such as Piper nigrum, Terminalia paniculata and Bauhinia purpurea on inflammation and oxidative stress in diabetic cardiomyopathy (DCM) which is induced by streptozotocin and nicotinamide administration in rats. We supplemented with PHF (250 and 500mg/kg/BW) to DCM rats for 45 days and evaluated their effects on oxidative stress markers, proinflammatory cytokines and mRNA expressions of the nuclear factor erythroid 2-related factor-2 (Nrf-2) and its linked genes (Heme oxygenase-1 (HO-1), superoxide dismutase (SOD), catalase) along with inflammatory genes (Tumour necrosis factor α (TNF-ɑ) and nuclear factor kappa B (NF-κB)). Our study demonstrated that PHF successfully attenuated inflammation and oxidative stress via mRNA up-regulation of Nrf-2, HO-1, SOD and catalase and concomitantly down-regulation of TNF-ɑ and NF-κB. Conversely, PHF also protected from hyperglycemia mediated cardiac-damage which was confirmed with histopathological and scanning electron microscopy analysis. In conclusion, our results suggested that PHF successfully ameliorated hyperglycemia mediated inflammation and oxidative stress via regulation of NF-κB/Nrf-2/HO-1 pathway. Therefore, these results recommend that PHF may be a prospective therapeutic agent for DCM.

中文翻译：

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多草药配方通过 NF-κB/Nrf-2/HO-1 通路减轻糖尿病大鼠的心脏炎症和氧化应激，从而改善糖尿病心肌病。

本研究旨在评估由胡椒、榄仁和紫荆等三种营养保健品制成的复方草药制剂 (PHF) 对链脲佐菌素和烟酰胺诱导的糖尿病心肌病 (DCM) 炎症和氧化应激的影响。老鼠。我们给 DCM 大鼠补充 PHF（250 和 500mg/kg/BW）45 天，并评估其对氧化应激标志物、促炎细胞因子和核因子红细胞 2 相关因子 2（Nrf-2）和 mRNA 表达的影响。其连锁基因（血红素加氧酶-1 (HO-1)、超氧化物歧化酶 (SOD)、过氧化氢酶）以及炎症基因（肿瘤坏死因子 α (TNF-ɑ) 和核因子 kappa B (NF-κB)）。我们的研究表明，PHF 通过上调 Nrf-2、HO-1、SOD 和过氧化氢酶的 mRNA 并同时下调 TNF-ɑ 和 NF-κB，成功减轻炎症和氧化应激。相反，PHF 还可以防止高血糖介导的心脏损伤，这一点已通过组织病理学和扫描电子显微镜分析得到证实。总之，我们的结果表明 PHF 通过调节 NF-κB/Nrf-2/HO-1 通路成功改善高血糖介导的炎症和氧化应激。因此，这些结果表明 PHF 可能是 DCM 的潜在治疗剂。