The fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is a major pest of maize crops and others row crops on the American continent, and this pest has spread to Africa and Asia. Such lepidopteran pest presents resistant populations to synthetic insecticides and to Bacillus thuringiensis (Bt) toxins and, consequently, new (bio) insecticides with distinct modes of action are required. In order to search for derivatives from Solanaceae as a potential source of bioactive compounds, a screening of 38 ethanolic extracts were prepared from 25 Solanaceae species. Using dietary exposure assessment, the ethanolic extracts from leaves of Acnistus arborescens (L.) Schltdl. and Datura stramonium L. were the most promising derivatives. A bioguided fractionation was performed with both plant species. The A. arborescens dichloromethane fraction was the most active, causing sublethal (growth inhibition) and lethal toxicity. Thus, this fraction was submitted to another fractionation procedure resulting in three subfractions (dichloromethane, ethyl acetate and methanol) that were tested against S. frugiperda caterpillars. All subfractions lead to significant sublethal effects, however, without significant mortality. The chemical diversity in the subfractions of A. arborescens was assessed through untargeted approaches, such as molecular networking and the in silico annotation tool NAP, by which two withanolides glycosides were annotated: (22R)-1-Oxo-3beta-(beta-D-glucopyranosyloxy)-14,20,22,27-tetrahydroxyergosta-5,24-diene-26-oic acid delta-lactone and withanoside XI. Therefore, derivatives of Solanaceae present promising compounds that may be useful in the framework of S. frugiperda integrated pest management. In addition, metabolomics is an innovative alternative and powerful approach to facilitate the putative identification of compounds in studies of natural products.

秋季粘虫，草地贪夜蛾（JE Smith）（鳞翅目：夜蛾科）是美洲大陆玉米作物和其他大田作物的主要害虫，这种害虫已传播​​到非洲和亚洲。这种鳞翅目害虫对合成杀虫剂和苏云金芽孢杆菌( Bt ) 毒素具有抗性，因此需要具有不同作用方式的新型（生物）杀虫剂。为了寻找茄科衍生物作为生物活性化合物的潜在来源，从 25 种茄科植物中筛选出 38 种乙醇提取物。使用膳食暴露评估，从Acnistus arborescens (L.) Schltdl 的叶子中提取乙醇提取物。和曼陀罗L. 是最有前途的衍生物。对两种植物物种进行生物引导分馏。所述A.藜二氯甲烷部分是最活跃的，引起亚致死（生长抑制）和致死毒性。因此，该级分被提交给另一个分馏程序，产生三个亚级分（二氯甲烷、乙酸乙酯和甲醇），用于抗草地贪夜蛾毛虫的测试。然而，所有亚组分都会导致显着的亚致死效应，但没有显着的死亡率。A. arborescens亚组分的化学多样性通过非靶向方法进行评估，例如分子网络和计算机注释工具 NAP，其中注释了两种 withanolides 苷：(22R)-1-Oxo-3beta-(beta-D-glupyranosyloxy)-14,20,22， 27-tetrahydroxyergosta-5,24-diene-26-oic acid δ-lactone 和 withanoside XI。因此，茄科的衍生物呈现出可用于草地贪夜蛾综合害虫管理框架中的有前景的化合物。此外，代谢组学是一种创新的替代和强大的方法，可促进天然产物研究中化合物的推定鉴定。