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Blocking Osa-miR1871 enhances rice resistance against Magnaporthe oryzae and yield
Plant Biotechnology Journal ( IF 13.8 ) Pub Date : 2021-11-02 , DOI: 10.1111/pbi.13743
Yan Li 1 , Ting-Ting Li 1 , Xiao-Rong He 1 , Yong Zhu 1 , Qin Feng 1 , Xue-Mei Yang 1 , Xin-Hui Zhou 1 , Guo-Bang Li 1 , Yun-Peng Ji 1 , Jing-Hao Zhao 1 , Zhi-Xue Zhao 1 , Mei Pu 1 , Shi-Xin Zhou 1 , Ji-Wei Zhang 1 , Yan-Yan Huang 1 , Jing Fan 1 , Wen-Ming Wang 1
Affiliation  

MicroRNAs (miRNAs) play vital roles in plant development and defence responses against various stresses. Here, we show that blocking miR1871 improves rice resistance against Magnaporthe oryzae and enhances grain yield simultaneously. The transgenic lines overexpressing miR1871 (OX1871) exhibit compromised resistance, suppressed defence responses and reduced panicle number resulting in slightly decreased yield. In contrast, the transgenic lines blocking miR1871 (MIM1871) show improved resistance, enhanced defence responses and significantly increased panicle number leading to enhanced yield per plant. The RNA-seq assay and defence response assays reveal that blocking miR1871 resulted in the enhancement of PAMP-triggered immunity (PTI). Intriguingly, miR1871 suppresses the expression of LOC_Os06g22850, which encodes a microfibrillar-associated protein (MFAP1) locating nearby the cell wall and positively regulating PTI responses. The mutants of MFAP1 resemble the phenotype of OX1871. Conversely, the transgenic lines overexpressing MFAP1 (OXMFAP1) or overexpressing both MFAP1 and miR1871 (OXMFAP1/OX1871) resemble the resistance of MIM1871. The time-course experiment data reveal that the expression of miR1871 and MFAP1 in rice leaves, panicles and basal internode is dynamic during the whole growth period to manipulate the resistance and yield traits. Our results suggest that miR1871 regulates rice yield and immunity via MFAP1, and the miR8171-MFAP1 module could be used in rice breeding to improve both immunity and yield.

中文翻译:

阻断 Osa-miR1871 可增强水稻对稻瘟病菌的抗性和产量

MicroRNAs (miRNAs) 在植物发育和针对各种胁迫的防御反应中发挥着至关重要的作用。在这里,我们展示了阻断 miR1871 可以提高水稻对稻瘟病菌的抗性并同时提高谷物产量。过表达 miR1871 (OX1871) 的转基因品系表现出抗性受损、防御反应受到抑制和穗数减少,导致产量略有下降。相比之下,阻断 miR1871 (MIM1871) 的转基因品系显示出提高的抗性、增强的防御反应和显着增加的穗数,从而提高了每株植物的产量。RNA-seq 分析和防御反应分析表明,阻断 miR1871 导致 PAMP 触发的免疫 (PTI) 增强。有趣的是,miR1871 抑制了LOC_Os06g22850 ,它编码位于细胞壁附近并正向调节 PTI 反应的微纤维相关蛋白 ( MFAP1 )。MFAP1的突变体类似于OX1871的表型。相反,过表达MFAP1 (OXMFAP1) 或过表达MFAP1和 miR1871 (OXMFAP1/OX1871) 的转基因系类似于 MIM1871 的抗性。时程实验数据表明,水稻叶片、穗部和基部节间的miR1871和MFAP1的表达在整个生育期内是动态的,以调控抗性和产量性状。我们的研究结果表明,miR1871 通过 MFAP1 调节水稻产量和免疫力而 miR8171 -MFAP1模块可用于水稻育种,提高免疫力和产量。
更新日期:2021-11-02
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