Kiwifruit (Actinidia chinensis Planch) is suitable for neutral acid soil. However, soil salinization is increasing in kiwifruit production areas, which has adverse effects on the growth and development of plants, leading to declining yields and quality. Therefore, analyzing the salt tolerance regulation mechanism can provide a theoretical basis for the industrial application and germplasm improvement of kiwifruit. We identified 120 NAC members and divided them into 13 subfamilies according to phylogenetic analysis. Subsequently, we conducted a comprehensive and systematic analysis based on the conserved motifs, key amino acid residues in the NAC domain, expression patterns, and protein interaction network predictions and screened the candidate gene AvNAC030. In order to study its function, we adopted the method of heterologous expression in Arabidopsis. Compared with the control, the overexpression plants had higher osmotic adjustment ability and improved antioxidant defense mechanism. These results suggest that AvNAC030 plays a positive role in the salt tolerance regulation mechanism in kiwifruit.

中文翻译：

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AvNAC030 是一种 NAC 域转录因子，可增强猕猴桃的盐胁迫耐受性

猕猴桃（Actinidia chinensis Planch）适合中性酸性土壤。然而，猕猴桃产区土壤盐渍化加剧，对植物的生长发育产生不利影响，导致产量和品质下降。因此，分析耐盐调控机制可为猕猴桃的产业化应用和种质改良提供理论依据。我们确定了 120 个 NAC 成员，并根据系统发育分析将它们分为 13 个亚科。随后，我们基于保守基序、NAC结构域中的关键氨基酸残基、表达模式和蛋白质相互作用网络预测进行了全面系统的分析，筛选出候选基因AvNAC030. 为了研究其功能，我们采用了拟南芥异源表达的方法。与对照相比，过表达植物具有更高的渗透调节能力和改善的抗氧化防御机制。这些结果表明AvNAC030在猕猴桃耐盐调节机制中发挥积极作用。