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Response surface methodology for the optimization of biophenols recovery from “alperujo” using supercritical fluid extraction. Comparison between Arbequina and Coratina cultivars
The Journal of Supercritical Fluids ( IF 3.9 ) Pub Date : 2021-10-30 , DOI: 10.1016/j.supflu.2021.105460
Cecilia Dauber 1 , Tatiana Carreras 1 , Adriana Fernández Fernández 1 , Bruno Irigaray 1 , Silvana Albores 2 , Adriana Gámbaro 1 , Elena Ibáñez 3 , Ignacio Vieitez 1
Affiliation  

Alpeorujo is a semi-solid residue from the olive oil industry that accounts for around 80–85% of the total processed olives; it contains phenolic compounds that could be used as natural preservatives in the food industry. The objective of this study was to optimize supercritical fluid extraction (SFE) of antioxidant compounds from alperujo using a Box-Behnken Design and response surface methodology. Antioxidant (ABTS and ORAC assays) and antimicrobial (MIC) potential of SFE extracts from Arbequina and Coratina were compared for extracts obtained in the optimal conditions to maximize the antioxidant activity (200 bar, 60 °C and 10% ethanol as modifier). Extracts from Coratina presented a significantly higher (p < 0.05) TPC (1487–2073 mg GAE/kg) and tocopherol content (345–454 ppm), although, in general, a correlation between these values and antioxidant activity of extracts was not observed. Moreover, Arbequina and Coratina SFE extracts showed moderate bacterial inhibitory potential against most of the bacteria studied.



中文翻译:

使用超临界流体萃取优化“alperujo”生物酚回收率的响应面方法。Arbequina 和 Coratina 品种之间的比较

Alpeorujo 是橄榄油行业的一种半固体残留物,约占加工橄榄总量的 80-85%;它含有酚类化合物,可在食品工业中用作天然防腐剂。本研究的目的是使用 Box-Behnken 设计和响应面方法优化 alperujo 中抗氧化化合物的超临界流体萃取 (SFE)。比较了来自 Arbequina 和 Coratina 的 SFE 提取物的抗氧化(ABTS 和 ORAC 测定)和抗微生物 (MIC) 潜力,以获得在最佳条件下获得的提取物,以最大限度地提高抗氧化活性(200 bar、60 °C 和 10% 乙醇作为改性剂)。Coratina 提取物的 TPC (1487–2073 mg GAE/kg) 和生育酚含量 (345–454 ppm) 显着更高 (p < 0.05),但总的来说,未观察到这些值与提取物的抗氧化活性之间存在相关性。此外,Arbequina 和 Coratina SFE 提取物对大多数研究的细菌显示出中等的细菌抑制潜力。

更新日期:2021-11-03
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