N⁶-methyladenosine (m⁶A) reader protein plays an important role in trichome morphology, developmental timing and morphogenesis in Arabidopsis. However, the function of m⁶A readers in plant-microbe interaction remains unclear. Here, a Malus YTH-domain family protein MhYTP2 was initially characterized as an m⁶A reader. MhYTP2 overexpression increased mRNA m⁶A modification level and translation efficiency. The m⁶A in the exon regions appeared to destabilize the mRNAs, whereas m⁶A in the untranslated regions positively correlated with the associated mRNA abundance. MhYTP2 overexpression enhanced apple powdery mildew resistance, possibly by rapidly degrading the bound mRNAs of MdMLO19 and MdMLO19-X1 and improving the translation efficiency of the antioxidant genes. To conclude, the results shed light on the apple m⁶A profile, the effect of MhYTP2 on m⁶A profile, and the m⁶A roles in MdMLO19 and MdMLO19-X1 mRNAs stability and glutamate dehydrogenase 1-like MdGDH1L mRNA translation efficiency.

中文翻译：

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m6A阅读器MhYTP2调节MdMLO19 mRNA稳定性和抗氧化基因翻译效率赋予苹果白粉病抗性

N ⁶ -甲基腺苷（m ^{6 A）阅读蛋白在}拟南芥的毛状体形态、发育时间和形态发生中起重要作用。然而，m ⁶ A 阅读器在植物-微生物相互作用中的作用仍不清楚。在这里，Malus YTH 域家族蛋白 MhYTP2 最初被定性为 m ⁶ A 阅读器。MhYTP2过表达增加了 mRNA m ⁶ A 修饰水平和翻译效率。外显子区域中的 m ⁶ A 似乎使 mRNA 不稳定，而非翻译区域中的 m ⁶ A 与相关的 mRNA 丰度呈正相关。MhYTP2过表达增强了苹果的白粉病抗性，可能是通过快速降解MdMLO19和MdMLO19-X1的结合 mRNA并提高抗氧化基因的翻译效率。总之，结果揭示了苹果 m ⁶ A 谱、MhYTP2 对 m ⁶ A 谱的影响，以及 m ⁶ A 在MdMLO19和MdMLO19-X1 mRNAs 稳定性和谷氨酸脱氢酶 1 样 MdGDH1L mRNA 翻译效率中的作用。