ABSTRACT

Purpose

In the present study, antifungal activity of ozonated olive oil (OZO) and ozonated distilled water (ODW) in the treatment of experimentally induced keratitis with C. albicans in rabbits were investigated.

Methods

The Groups were composed of as 1, 2, 3, 4, 5, 7 (n = 5 rabbits, 10 eyes/in each group) and Group 6 (n = 10 rabbits, 20 eyes/in the group). Fourty-eight hours after C. albicans inoculation; Group 1 received fluconazle (FLU)+OZO drops, Group 2 received FLU drop, Group 3 received OZO drop, Group 4 received FLU+ODW drops, Group 5 received ODW drop, Group 6 (infected control group) and Group 7 received PBS drop (negative control group). Treatment continued in all groups for 22 days for every 8 hours.

Results

Cornea cultures made 24 days post inoculation revealed statistically significant differences (p < 0,05) with concern to C. albicans amounts between Group 6 and Group 1–5. Statistical comparison of corneal opacity and corneal ulcer and conjunctivitis values among the Group 6 and Group 1–5 were also different significantly (p < 0,05) on days 20 and 24 post inoculation.

Conclusion

OZO and ODW were found to be effective in treating C. albicans keratitis in the present study. It has also been proven by this study that ODW contain 26 μg/ml was the most effective in the treatment of C. albicans keratitis.

中文翻译：

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臭氧疗法治疗兔实验性白念珠菌性角膜炎疗效研究

摘要

目的

在本研究中，研究了臭氧化橄榄油 (O​​ZO) 和臭氧化蒸馏水 (ODW) 在治疗白念珠菌实验性角膜炎中的抗真菌活性。

方法

组由1、2、3、4、5、7（n = 5只兔子，10只眼/每组）和第6组（n = 10只兔子，20只眼睛/每组）组成。白色念珠菌接种后四十八小时；第 1 组接受氟康唑 (FLU)+OZO 滴剂，第 2 组接受 FLU 滴剂，第 3 组接受 OZO 滴剂，第 4 组接受 FLU+ODW 滴剂，第 5 组接受 ODW 滴剂，第 6 组（感染对照组）和第 7 组接受 PBS 滴剂(阴性对照组)。所有组的治疗持续 22 天，每 8 小时一次。

结果

接种后 24 天进行的角膜培养显示，第 6 组和第 1-5 组之间的白色念珠菌数量存在统计学显着差异 ( p < 0,05) 。在接种后第 20 天和第 24 天，第 6 组和第 1-5 组之间角膜混浊和角膜溃疡和结膜炎值的统计比较也有显着差异 ( p < 0,05)。

结论

在本研究中发现 OZO 和 ODW 可有效治疗白色念珠菌角膜炎。本研究还证明，含有 26 μg/ml 的 ODW 对治疗白色念珠菌角膜炎最有效。