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Proteomic analysis of the host–pathogen interface in experimental cholera
Nature Chemical Biology ( IF 14.8 ) Pub Date : 2021-10-21 , DOI: 10.1038/s41589-021-00894-4
Abdelrahim Zoued 1, 2, 3 , Hailong Zhang 1, 2, 3 , Ting Zhang 1, 2 , Rachel T Giorgio 2 , Carole J Kuehl 1, 2, 3 , Bolutife Fakoya 1, 2 , Brandon Sit 1, 2 , Matthew K Waldor 1, 2, 3
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The microbial cell surface is a site of critical microbe–host interactions that often control infection outcomes. Defining the set of host proteins present at this interface has been challenging. Here we used a surface-biotinylation approach coupled to quantitative mass spectrometry to identify and quantify both bacterial and host proteins present on the surface of diarrheal fluid-derived Vibrio cholerae in an infant rabbit model of cholera. The V. cholerae surface was coated with numerous host proteins, whose abundance were driven by the presence of cholera toxin, including the C-type lectin SP-D. Mice lacking SP-D had enhanced V. cholerae intestinal colonization, and SP-D production shaped both host and pathogen transcriptomes. Additional host proteins (AnxA1, LPO and ZAG) that bound V. cholerae were also found to recognize distinct taxa of the murine intestinal microbiota, suggesting that these host factors may play roles in intestinal homeostasis in addition to host defense.



中文翻译:

实验性霍乱宿主-病原体界面的蛋白质组学分析

微生物细胞表面是通常控制感染结果的关键微生物-宿主相互作用的部位。定义存在于该界面的宿主蛋白集一直具有挑战性。在这里,我们使用与定量质谱联用的表面生物素化方法来识别和量化存在于霍乱婴儿兔模型中腹泻液衍生的霍乱弧菌表面的细菌和宿主蛋白质。 霍乱弧菌表面涂有许多宿主蛋白,其丰度是由霍乱毒素的存在驱动的,包括 C 型凝集素 SP-D。缺乏 SP-D 的小鼠具有增强的霍乱弧菌肠道定植和 SP-D 的产生塑造了宿主和病原体的转录组。还发现结合霍乱弧菌的其他宿主蛋白(AnxA1、LPO 和 ZAG)可识别小鼠肠道微生物群的不同分类群,这表明这些宿主因子除了宿主防御外,还可能在肠道稳态中发挥作用。

更新日期:2021-10-21
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