Daphnia magna belongs to the Cladocera and plays an important role in the water ecosystem. With the intensification of water pollution, the wild population of D. magna has declined rapidly in recent years, and insufficient molecular markers have limited effective research and conservation of this species. In this research, 30 novel single nucleotide polymorphism (SNP) markers were developed in a cultivar of D. magna and 12 wild D. magna using restriction site-associated DNA sequencing (RAD-seq). The minor allele frequency, observed heterozygosity, and expected heterozygosity ranged from 0.115 to 0.483, 0.073 to 0.800, and 0.077 to 0.520, respectively. The PIC ranged from 0.071 to 0.403. Six loci showed significant deviations from the Hardy-Weinberg equilibrium after Bonferroni correction (p < 0.05).These newly developed polymorphic SNP markers for D. magna are of great significance in terms of the genetic breeding of D. magna, identification of wild and artificially domesticated species and conservation genetics research.

Daphnia magna属于枝角类，在水生态系统中占有重要地位。近年来，随着水体污染的加剧，大戟野生种群数量急剧下降，分子标记不足限制了对该物种的有效研究和保护。在这项研究中，30 个新的单核苷酸多态性 (SNP) 标记在D. magna栽培品种和 12 个野生D. magna 中开发使用限制性位点相关 DNA 测序 (RAD-seq)。次要等位基因频率、观察到的杂合度和预期的杂合度分别介于 0.115 到 0.483、0.073 到 0.800 和 0.077 到 0.520 之间。PIC 介于 0.071 至 0.403 之间。6个位点表现出从Hardy-Weinberg平衡显著偏差Bonferroni校正（P <0.05）。这些后新开发的多态性SNP标记D.麦格纳都具有重要意义的遗传育种方面的大型溞，野生和人工识别驯化物种和保护遗传学研究。