The use of natural extracts in medical treatments has grown in recent years. However, conventional extraction methodologies generate large amounts of toxic residues, which cause severe problems for human health and the environment. For this reason, in this study, the pressurized liquid extraction method was used to obtain chlorophyll-rich spinach extracts applicable for Antimicrobial Photodynamic Therapy, a medical modality aimed at pathological conditions caused by microorganisms. Several conditions were evaluated, with variations in temperature, pressure, and percentage of ethanol in water as solvent. The highest yield was achieved by the combination of 8 MPa pressure, 75 °C, and 80% (v/v) ethanol/water, for which we obtained a yield of 25.3% (w/w). The extraction of kinetic curves fitted Sovová's model. The clean methodology belongs to the green chemistry and has proven to be fast and efficient. The extract obtained has a high potential for the development of phytotherapeutic medicines.

近年来，天然提取物在医学治疗中的使用有所增加。然而，传统的提取方法会产生大量有毒残留物，对人类健康和环境造成严重问题。因此，在本研究中，采用加压液体提取方法获得适用于抗微生物光动力疗法的富含叶绿素的菠菜提取物，这是一种针对微生物引起的病理状况的医疗方式。评估了几种条件，温度、压力和作为溶剂的水中乙醇百分比的变化。最高产率是通过 8 MPa 压力、75 °C 和 80% (v/v) 乙醇/水的组合实现的，我们获得了 25.3% (w/w) 的产率。动力学曲线的提取符合 Sovová 的模型。清洁方法属于绿色化学，已被证明是快速有效的。获得的提取物具有开发植物治疗药物的巨大潜力。