The present research can be considered as a proof-of-principle study focused on the determination of chiral pesticides using a supercritical fluid extraction instrument coupled on-line with an enantioselective supercritical fluid chromatography-triple quadrupole mass spectrometry. To the best of Authors’ knowledge, this is the first description of an on-line approach for the extraction and determination of chiral pesticides. Metalaxyl, benalaxyl and dimethenamid were investigated in nine hemp seed samples belonging to four varieties of Cannabis sativa; only in one case a pesticide was found at levels above the method limit of quantification (LoQ), though within the EU maximum residue level value. The figures-of-merit determined were linearity, precision, limit of detection (LoD), and LoQ. Regression coefficients were between 0.9856 and 0.9973, the LoDs were in the 0.04–0.41 μg kg⁻¹ range, the LoQs were in the 0.12–1.38 μg kg⁻¹ range, while coefficients of variation were between 1 and 3% (10 μg kg⁻¹ level).

本研究可被视为一项原理验证研究，重点是使用超临界流体萃取仪与对映选择性超临界流体色谱-三重四极杆质谱联用在线测定手性农药。据作者所知，这是对手性农药提取和测定的在线方法的首次描述。在属于四个大麻品种的九个大麻种子样品中研究了甲霜灵、苯霜灵和噻吩草胺; 仅在一个案例中发现农药的水平高于方法定量限 (LoQ)，但在欧盟最大残留水平值范围内。确定的品质因数包括线性、精密度、检测限 (LoD) 和 LoQ。回归系数在 0.9856 和 0.9973 之间，LoD 在 0.04-0.41 μg kg ^-1范围内，LoQ 在 0.12-1.38 μg kg ^-1范围内，而变异系数在 1% 和 3% 之间（10 μg kg ^{-1 −1}级）。