Holder pasteurization is the current recommended method for donor human milk treatment. This method effectively eliminates most life-threatening contaminants in donor milk, but it also greatly reduces some of its biological properties. Consequently, there is a growing interest for developing novel processing methods that can ensure both microbial inactivation and a higher retention of the functional components of donor milk. Our aim was to offer a comprehensive overview of the analytical techniques available for the evaluation of such methods. To suggest an efficient workflow for the analysis of processed donor milk, a safety analytical panel as well as a nutritional value and functionality analytical panel are discussed, together with the principles, benefits, and drawbacks of the available techniques. Concluding on the suitability of a novel method requires a multifactorial approach which can be achieved by a combination of analytical targets and by using complementary assays to cross-validate the obtained results.

支架巴氏杀菌是目前推荐的供体母乳处理方法。这种方法有效地消除了捐赠乳中大多数危及生命的污染物，但也大大降低了其一些生物学特性。因此，人们对开发新的加工方法越来越感兴趣，这种方法可以确保微生物灭活并更好地保留供体乳的功能成分。我们的目标是全面概述可用于评估此类方法的分析技术。为了提出用于分析加工捐赠乳的有效工作流程，讨论了安全分析面板以及营养价值和功能分析面板，以及可用技术的原理、优点和缺点。